TY - JOUR
T1 - CDNA cloning and characterization of the third isoform of human peroxin Pex11p
AU - Tanaka, Atsushi
AU - Okumoto, Kanji
AU - Fujiki, Yukio
N1 - Funding Information:
We thank S. Mukai for RT products from human fibroblasts and the other members of our laboratory for discussion. This work was supported in part by SORST grant (to Y.F.) from the Science and Technology Corporation of Japan; Grants-in-Aid for Scientific Research (12308033, 12557017, 12206069, 13206060, and 14037253 to Y.F.) and Grant of National Project on Protein Structural and Functional Analyses (to Y.F.) from The Ministry of Education, Culture, Sports, Science and Technology of Japan; a grant from Japan Foundation for Applied Enzymology. K.O. is a postdoctoral fellow of the Japan Society for the Promotion of Science.
PY - 2003/1/24
Y1 - 2003/1/24
N2 - We have cloned a human cDNA encoding an isoform of the peroxin Pex11p, termed Pex11pγ, by BLASTP homology search on eukaryotic protein database and reverse transcription (RT)-PCR of human fibroblast RNA. Pex11pγ was 241 amino-acid long, with two putative transmembrane segments, showing 22% and 23% amino-acid identity to Pex11pα and Pex11pβ, respectively. PEX11γ gene was located on chromosome 19, distinct from PEX11α and PEX11β. Pex11pγ was found to be a peroxisomal membrane protein, as assessed by colocalization with peroxisome targeting signal type 1 (PTS1)-proteins, in epitope-tagged Pex11pγ-expressing Chinese hamster ovary cells. Pex11pγ exposes both of the N- and C-terminal parts to the cytosol. PEX11γ was not induced in rats by treatment of clofibrate, a peroxisome proliferator, similar to constitutively expressed PEX11β but in contrast to inducible PEX11α.
AB - We have cloned a human cDNA encoding an isoform of the peroxin Pex11p, termed Pex11pγ, by BLASTP homology search on eukaryotic protein database and reverse transcription (RT)-PCR of human fibroblast RNA. Pex11pγ was 241 amino-acid long, with two putative transmembrane segments, showing 22% and 23% amino-acid identity to Pex11pα and Pex11pβ, respectively. PEX11γ gene was located on chromosome 19, distinct from PEX11α and PEX11β. Pex11pγ was found to be a peroxisomal membrane protein, as assessed by colocalization with peroxisome targeting signal type 1 (PTS1)-proteins, in epitope-tagged Pex11pγ-expressing Chinese hamster ovary cells. Pex11pγ exposes both of the N- and C-terminal parts to the cytosol. PEX11γ was not induced in rats by treatment of clofibrate, a peroxisome proliferator, similar to constitutively expressed PEX11β but in contrast to inducible PEX11α.
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U2 - 10.1016/S0006-291X(02)02936-4
DO - 10.1016/S0006-291X(02)02936-4
M3 - Article
C2 - 12559946
AN - SCOPUS:0037462961
SN - 0006-291X
VL - 300
SP - 819
EP - 823
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -