TY - JOUR
T1 - Cdh1 is an antagonist of the spindle assembly checkpoint
AU - Nagai, Masayoshi
AU - Ushimaru, Takashi
N1 - Funding Information:
We thank Clarence Chan, Orna Cohen-Fix, Johannes Hegemann, Hisao Moriya, Andrew Murray, Kim Nasmyth, Wolfgang Seufert, Uttam Surana, and Akio Toh-e for valuable materials. We thank laboratory members of T.U. and Ayumu Yamamoto for helpful discussions and reading of the manuscript. This work was supported in part by Grants-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Science, Sports, and Culture of Japan (No. 19370082 and 23570225 ).
PY - 2014/10
Y1 - 2014/10
N2 - The spindle assembly checkpoint (SAC) monitors unsatisfied connections of microtubules to kinetochores and prevents anaphase onset by inhibition of the ubiquitin ligase E3 anaphase-promoting complex or cyclosome (APC/C) in association with the activator Cdc20. Another APC/C activator, Cdh1, exists permanently throughout the cell cycle but it becomes active from telophase to G1. Here, we show that Cdh1 is partially active and mediates securin degradation even in SAC-active metaphase cells. Additionally, Cdh1 mediates Cdc20 degradation in metaphase, promoting formation of the APC/C-Cdh1. These results indicate that Cdh1 opposes the SAC and promotes anaphase transition.
AB - The spindle assembly checkpoint (SAC) monitors unsatisfied connections of microtubules to kinetochores and prevents anaphase onset by inhibition of the ubiquitin ligase E3 anaphase-promoting complex or cyclosome (APC/C) in association with the activator Cdc20. Another APC/C activator, Cdh1, exists permanently throughout the cell cycle but it becomes active from telophase to G1. Here, we show that Cdh1 is partially active and mediates securin degradation even in SAC-active metaphase cells. Additionally, Cdh1 mediates Cdc20 degradation in metaphase, promoting formation of the APC/C-Cdh1. These results indicate that Cdh1 opposes the SAC and promotes anaphase transition.
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U2 - 10.1016/j.cellsig.2014.07.007
DO - 10.1016/j.cellsig.2014.07.007
M3 - Article
C2 - 25025567
AN - SCOPUS:84904489175
SN - 0898-6568
VL - 26
SP - 2217
EP - 2222
JO - Cellular Signalling
JF - Cellular Signalling
IS - 10
ER -