TY - JOUR
T1 - Cdh1 degradation is mediated by APC/C–Cdh1 and SCF–Cdc4 in budding yeast
AU - Nagai, Masayoshi
AU - Shibata, Atsuko
AU - Ushimaru, Takashi
N1 - Funding Information:
We thank Akio Toh-e, Erin O'Shea, Jürgen Dohmen, Kim Nasmyth, Miki Shinohara, Orna Cohen-Fix, Stephan Irniger, Stephen Elledge, Uttam Surana, Wolfgang Seufert, and Yoshinori Watanabe for generous gifts of materials, and laboratory members of TU, Ayumu Yamamoto and Takanori Oyoshi for helpful discussions. This work was supported in part by The Japan Society for the Promotion of Science (JSPS) (grant No. 19370082 and 23570225 to TU).
Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2018/12/2
Y1 - 2018/12/2
N2 - Cdh1, a substrate-recognition subunit of anaphase-promoting complex/cyclosome (APC/C), is a tumor suppressor, and it is downregulated in various tumor cells in humans. APC/C–Cdh1 is activated from late M phase to G1 phase by antagonizing Cdk1-mediated inhibitory phosphorylation. However, how Cdh1 protein levels are properly regulated is ill-defined. Here we show that Cdh1 is degraded via APC/C–Cdh1 and Skp1–Cullin1–F-box (SCF)–Cdc4 in the budding yeast Saccharomyces cerevisiae. Cdh1 degradation was promoted by forced localization of Cdh1 into the nucleus, where APC/C and SCF are present. Cdk1 promoted APC/C–Cdh1-mediated Cdh1 degradation, whereas polo kinase Cdc5 elicited SCF–Cdc4-mediated degradation. Thus, Cdh1 degradation is controlled via multiple pathways.
AB - Cdh1, a substrate-recognition subunit of anaphase-promoting complex/cyclosome (APC/C), is a tumor suppressor, and it is downregulated in various tumor cells in humans. APC/C–Cdh1 is activated from late M phase to G1 phase by antagonizing Cdk1-mediated inhibitory phosphorylation. However, how Cdh1 protein levels are properly regulated is ill-defined. Here we show that Cdh1 is degraded via APC/C–Cdh1 and Skp1–Cullin1–F-box (SCF)–Cdc4 in the budding yeast Saccharomyces cerevisiae. Cdh1 degradation was promoted by forced localization of Cdh1 into the nucleus, where APC/C and SCF are present. Cdk1 promoted APC/C–Cdh1-mediated Cdh1 degradation, whereas polo kinase Cdc5 elicited SCF–Cdc4-mediated degradation. Thus, Cdh1 degradation is controlled via multiple pathways.
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U2 - 10.1016/j.bbrc.2018.10.179
DO - 10.1016/j.bbrc.2018.10.179
M3 - Article
C2 - 30396569
AN - SCOPUS:85055912267
SN - 0006-291X
VL - 506
SP - 932
EP - 938
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -