TY - JOUR
T1 - CD25+CD4+ regulatory T cells exert in vitro suppressive activity independent of CTLA-4
AU - Kataoka, Hiroshi
AU - Takahashi, Shigekazu
AU - Takase, Kan
AU - Yamasaki, Sho
AU - Yokosuka, Tadashi
AU - Koike, Takao
AU - Saito, Takashi
N1 - Funding Information:
We thank H. Arase for discussion, M. Sakuma and R. Shiina for experimental assistance, and H. Yamaguchi for secretarial assistance. This work was supported by grants from the Ministry of Education, Culture, Sports, Science, and Technology, Japan.
PY - 2005/4
Y1 - 2005/4
N2 - Cytotoxic T lymphocyte antigen-4 (CTLA-4) is constitutively expressed on CD25+CD4+ regulatory T cells (Treg) and is suggested to play a role in Treg-mediated suppression. However, the results of analysis with anti-CTLA-4 have been controversial. We addressed this issue by analyzing mice over-expressing or deficient in CTLA-4. For over-expression, CTLA-4 transgenic mice expressing a full-length (FL) or a truncated (TL) mutant of CTLA-4 were analyzed. FL T cells expressed similar levels of CTLA-4 to Treg, whereas TL T cells expressed much higher levels on the cell surface. The number of Treg in both mice was decreased, although Foxp3 expression was not altered. Treg from both mice exerted suppressive activity, whereas CD25- T cells from FL mice showed no suppression. Furthermore, CD25+CD4 thymocytes from young CTLA-4-deficient mice were analyzed and found to exhibit suppressive activity. These results indicate that Treg exert in vitro suppressive activity independent of CTLA-4 expression.
AB - Cytotoxic T lymphocyte antigen-4 (CTLA-4) is constitutively expressed on CD25+CD4+ regulatory T cells (Treg) and is suggested to play a role in Treg-mediated suppression. However, the results of analysis with anti-CTLA-4 have been controversial. We addressed this issue by analyzing mice over-expressing or deficient in CTLA-4. For over-expression, CTLA-4 transgenic mice expressing a full-length (FL) or a truncated (TL) mutant of CTLA-4 were analyzed. FL T cells expressed similar levels of CTLA-4 to Treg, whereas TL T cells expressed much higher levels on the cell surface. The number of Treg in both mice was decreased, although Foxp3 expression was not altered. Treg from both mice exerted suppressive activity, whereas CD25- T cells from FL mice showed no suppression. Furthermore, CD25+CD4 thymocytes from young CTLA-4-deficient mice were analyzed and found to exhibit suppressive activity. These results indicate that Treg exert in vitro suppressive activity independent of CTLA-4 expression.
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U2 - 10.1093/intimm/dxh221
DO - 10.1093/intimm/dxh221
M3 - Article
C2 - 15724061
AN - SCOPUS:17644376164
SN - 0953-8178
VL - 17
SP - 421
EP - 427
JO - International immunology
JF - International immunology
IS - 4
ER -