TY - JOUR
T1 - Cbx2, a polycomb group gene, is required for Sry gene expression in mice
AU - Katoh-Fukui, Yuko
AU - Miyabayashi, Kanako
AU - Komatsu, Tomoko
AU - Owaki, Akiko
AU - Baba, Takashi
AU - Shima, Yuichi
AU - Kidokoro, Tomohide
AU - Kanai, Yoshiakira
AU - Schedl, Andreas
AU - Wilhelm, Dagmar
AU - Koopman, Peter
AU - Okuno, Yasushi
AU - Morohashi, Ken Ichirou
PY - 2012/2
Y1 - 2012/2
N2 - Mice lacking the function of the polycomb group protein CBX2 (chromobox homolog 2; also known as M33) show defects in gonadal, adrenal, and splenic development. In particular, XY knockout (KO) mice develop ovaries but not testes, and the gonads are hypoplastic in both sexes. However, how CBX2 regulates development of these tissues remains largely unknown. In the present study, weused microarray, RT-PCR, and immunohistochemical analyses to show that the expression of Sry, Sox9, Lhx9, Ad4BP/SF-1, Dax-1, Gata4, Arx, and Dmrt1, genes encoding transcription factors essential for gonadal development, is affected in Cbx2 KO gonads. Male-to-female sex reversal in Cbx2 KO mice was rescued by crossing them with transgenic mice displaying forced expression of Sry or Sox9. However, testes remained hypoplastic in these mice, indicating that the size and the sex of the gonad are determined by different sets of genes. Our study implicates Cbx2 in testis differentiation through regulating Sry gene expression.
AB - Mice lacking the function of the polycomb group protein CBX2 (chromobox homolog 2; also known as M33) show defects in gonadal, adrenal, and splenic development. In particular, XY knockout (KO) mice develop ovaries but not testes, and the gonads are hypoplastic in both sexes. However, how CBX2 regulates development of these tissues remains largely unknown. In the present study, weused microarray, RT-PCR, and immunohistochemical analyses to show that the expression of Sry, Sox9, Lhx9, Ad4BP/SF-1, Dax-1, Gata4, Arx, and Dmrt1, genes encoding transcription factors essential for gonadal development, is affected in Cbx2 KO gonads. Male-to-female sex reversal in Cbx2 KO mice was rescued by crossing them with transgenic mice displaying forced expression of Sry or Sox9. However, testes remained hypoplastic in these mice, indicating that the size and the sex of the gonad are determined by different sets of genes. Our study implicates Cbx2 in testis differentiation through regulating Sry gene expression.
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U2 - 10.1210/en.2011-1055
DO - 10.1210/en.2011-1055
M3 - Article
C2 - 22186409
AN - SCOPUS:84856113820
SN - 0013-7227
VL - 153
SP - 913
EP - 924
JO - Endocrinology
JF - Endocrinology
IS - 2
ER -