Catalytic activity of Laccase hosted in reversed micelles

Junji Michizoe, Masahiro Goto, Shintaro Furusaki

    Research output: Contribution to journalArticlepeer-review

    39 Citations (Scopus)


    Nanostructured reversed micelles induce a high laccase activity in organic solvents, because enzymes can maintain their highly dimensional structure in water pools of reversed micelles [RMs]. Laccase attracts considerable attention as a novel industrial enzyme due to its high capability to catalyze the oxidation of aromatic compounds. The catalytic activities of lyophilized laccase and laccase entrapped in RMs were compared using an oxidative reaction. Laccase hosted in an anionic RM effectively catalyzed the oxidative reaction in various organic solvents, while lyophilized laccase exhibited no such catalytic activity. To optimize the Preparation and reaction conditions for laccase in RMs, we examined the effects of pH of water pools of RMs, the concentrations of both enzyme and surfactant for the preparation of RMs, the hydration ratio (Wo), and the reaction temperature on laccase catalytic activity in organic media. Laccase entrapped in RMs exhibited the highest catalytic activity in isooctane under the following conditions: bis-2-ethylhexyl sulfosuccinate sodium salt (AOT) of 100 mM, pH 6.0, Wo=40, and reaction temperature of 60°C. Under the optimum conditions, environmental pollutants such as bisphenol A, 2,4-dichlorophenol and 2,4,6-trichlorophenol were effectively degraded in 3 h.

    Original languageEnglish
    Pages (from-to)67-71
    Number of pages5
    JournalJournal of Bioscience and Bioengineering
    Issue number1
    Publication statusPublished - 2001

    All Science Journal Classification (ASJC) codes

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology


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