Ca2+-influx gated by inositol-3,4,5,6-tetrakisphosphate in NIH/3T3 fibroblasts

Minako Hashii, Masato Hirata, Shoichiro Ozaki, Yoshinori Nozawa, Haruhiro Higashida

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10 Citations (Scopus)


The functional role of three inositol tetrakisphosphate isomers on Ca2+ influx was examined by intracellular application in NIH/3T3 mouse fibroblasts. Infusion of inositol-1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4), inositol-1,3,4,6-tetrakisphosphate, and inositol-3,4,5,6-tetrakisphosphate (Ins(3,4,5,6)P4) from the patch pipette into the cytoplasm produced a transient increase in cytoplasmic free Ca2+ concentration by fura-2 recording in extracellular Ca2+-dependent fashion. Stimulation of NIH/3T3 cells with bradykinin increased the levels of Ins(1,3,4,5)P4 and Ins(3,4,5,6)P4. These results suggest that mouse fibroblast cells possess a Ca2+ influx pathway gated with naturally occuring inositol tetrakisphosphates in response to stimulation with bradykinin.

Original languageEnglish
Pages (from-to)1300-1306
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - May 15 1994

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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