Casein kinase 2 is essential for mitophagy

Tomotake Kanki; Yusuke Kurihara; Xiulian Jin; Tadahiro Goda; Yusuke Ono; Masamune Aihara; Yuko Hirota; Tetsu Saigusa; Yoshimasa Aoki; Takeshi Uchiumi; Dongchon Kang

doi:10.1038/embor.2013.114

Casein kinase 2 is essential for mitophagy

Tomotake Kanki, Yusuke Kurihara, Xiulian Jin, Tadahiro Goda, Yusuke Ono, Masamune Aihara, Yuko Hirota, Tetsu Saigusa, Yoshimasa Aoki, Takeshi Uchiumi, Dongchon Kang

Research output: Contribution to journal › Article › peer-review

115 Citations (Scopus)

Abstract

Mitophagy is a process that selectively degrades mitochondria. When mitophagy is induced in yeast, the mitochondrial outer membrane protein Atg32 is phosphorylated, interacts with the adaptor protein Atg11 and is recruited into the vacuole with mitochondria. We screened kinase-deleted yeast strains and found that CK2 is essential for Atg32 phosphorylation, Atg32-Atg11 interaction and mitophagy. Inhibition of CK2 specifically blocks mitophagy, but not macroautophagy, pexophagy or the Cvt pathway. In vitro, CK2 phosphorylates Atg32 at serine 114 and serine 119. We conclude that CK2 regulates mitophagy by directly phosphorylating Atg32.

Original language	English
Pages (from-to)	788-794
Number of pages	7
Journal	EMBO Reports
Volume	14
Issue number	9
DOIs	https://doi.org/10.1038/embor.2013.114
Publication status	Published - Sept 2013

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Genetics

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10.1038/embor.2013.114

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title = "Casein kinase 2 is essential for mitophagy",

abstract = "Mitophagy is a process that selectively degrades mitochondria. When mitophagy is induced in yeast, the mitochondrial outer membrane protein Atg32 is phosphorylated, interacts with the adaptor protein Atg11 and is recruited into the vacuole with mitochondria. We screened kinase-deleted yeast strains and found that CK2 is essential for Atg32 phosphorylation, Atg32-Atg11 interaction and mitophagy. Inhibition of CK2 specifically blocks mitophagy, but not macroautophagy, pexophagy or the Cvt pathway. In vitro, CK2 phosphorylates Atg32 at serine 114 and serine 119. We conclude that CK2 regulates mitophagy by directly phosphorylating Atg32.",

author = "Tomotake Kanki and Yusuke Kurihara and Xiulian Jin and Tadahiro Goda and Yusuke Ono and Masamune Aihara and Yuko Hirota and Tetsu Saigusa and Yoshimasa Aoki and Takeshi Uchiumi and Dongchon Kang",

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T1 - Casein kinase 2 is essential for mitophagy

AU - Kanki, Tomotake

AU - Kurihara, Yusuke

AU - Jin, Xiulian

AU - Goda, Tadahiro

AU - Ono, Yusuke

AU - Aihara, Masamune

AU - Hirota, Yuko

AU - Saigusa, Tetsu

AU - Aoki, Yoshimasa

AU - Uchiumi, Takeshi

AU - Kang, Dongchon

PY - 2013/9

Y1 - 2013/9

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AB - Mitophagy is a process that selectively degrades mitochondria. When mitophagy is induced in yeast, the mitochondrial outer membrane protein Atg32 is phosphorylated, interacts with the adaptor protein Atg11 and is recruited into the vacuole with mitochondria. We screened kinase-deleted yeast strains and found that CK2 is essential for Atg32 phosphorylation, Atg32-Atg11 interaction and mitophagy. Inhibition of CK2 specifically blocks mitophagy, but not macroautophagy, pexophagy or the Cvt pathway. In vitro, CK2 phosphorylates Atg32 at serine 114 and serine 119. We conclude that CK2 regulates mitophagy by directly phosphorylating Atg32.

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Casein kinase 2 is essential for mitophagy

Abstract

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