TY - JOUR
T1 - Bystander-killing effect and cyclic induction of TNF-α gene under heat-inducible promoter gadd 153
AU - Ito, Akira
AU - Shinkai, Masashige
AU - Bouhon, Isabelle Anne
AU - Honda, Hiroyuki
AU - Kobayashi, Takeshi
N1 - Funding Information:
We are grateful to Prof. Nikki Holbrook (National Institute on Aging, Baltimore, MD, USA) for providing the JymCATO plasmid carrying the gadd 153 promoter and Dr. Hirohumi Hamada (Cancer Chemotherapy Center, Japanese Foundation for Cancer Research) for providing the plasmid SKhTNF-(U carrying the hTNF-a gene. This study was partially funded by a Grant-in-Aid for Scientific Research on Priority Areas (No. 10145104) and by a Grant-in-Aid for JSPS Fellows (No. 12004238) from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2000/10
Y1 - 2000/10
N2 - The bystander-killing effect and cyclic induction of tumor necrosis factor (TNF)-a gene expression under the heat-inducible promoter gadd 153 (growth arrest and DNA damage) were investigated. The plasmid harboring the TNF-a gene under the control of the gadd 153 promoter (pGadTNF) was introduced into cells of the human glioma cell line U251-SP. When the transfected cells were exposed to heat treatment, TNF-a gene expression was induced. The heated pGadTNF-transfected cells were co-cultured the nonheated pGadTNF-transfected cells as bystander cells. As a result, TNF-a gene expression in the bystander cells was observed. The level of TNF-a gene expression was further enhanced in the co-cultured cells. Due to this cyclic induction, a strong cytotoxic effect on the bystander cells was observed. Based on this bystander-killing effect, it was concluded that the transfection of the TNF-a gene under the control of the gadd 153 promoter into tumor cells combined with hyperthermia would be a potent tool for cancer therapy.
AB - The bystander-killing effect and cyclic induction of tumor necrosis factor (TNF)-a gene expression under the heat-inducible promoter gadd 153 (growth arrest and DNA damage) were investigated. The plasmid harboring the TNF-a gene under the control of the gadd 153 promoter (pGadTNF) was introduced into cells of the human glioma cell line U251-SP. When the transfected cells were exposed to heat treatment, TNF-a gene expression was induced. The heated pGadTNF-transfected cells were co-cultured the nonheated pGadTNF-transfected cells as bystander cells. As a result, TNF-a gene expression in the bystander cells was observed. The level of TNF-a gene expression was further enhanced in the co-cultured cells. Due to this cyclic induction, a strong cytotoxic effect on the bystander cells was observed. Based on this bystander-killing effect, it was concluded that the transfection of the TNF-a gene under the control of the gadd 153 promoter into tumor cells combined with hyperthermia would be a potent tool for cancer therapy.
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U2 - 10.1016/S1389-1723(01)80015-3
DO - 10.1016/S1389-1723(01)80015-3
M3 - Article
C2 - 16232886
AN - SCOPUS:0034302595
SN - 1389-1723
VL - 90
SP - 437
EP - 441
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 4
ER -
