TY - GEN
T1 - Bundle formation in a crowded environment with giant DNA and F-actin inside a micro-meter scale closed-space
AU - Negishi, Makiko
AU - Sakaue, Takahiro
AU - Taniguchi, Kingo
AU - Yoshikawa, Kenichi
PY - 2008
Y1 - 2008
N2 - It is known that actin play the essential role in the regulation of the structure and function in cellular and cell nuclei systems. DNA and actin filaments are the two major components of cell nuclei. We developed an artificial cell nuclei model with T4 DNA and F-actin in phospholipid-coated micro-meter scale closed spheres (MPDs). In a MPD, the mixture of T4 DNA and F-actin was found to phase-separate into F-actin phase (Px) and T4 DNA rich phase (P2), where the manner of the segregation is dependent on the total volume fraction of F-actin and T4 DNA. In addition, we found that, in a MPD, T4 DNA forms bundles in a crowded micro-space with F-actin even in the absence of multivalent cations.
AB - It is known that actin play the essential role in the regulation of the structure and function in cellular and cell nuclei systems. DNA and actin filaments are the two major components of cell nuclei. We developed an artificial cell nuclei model with T4 DNA and F-actin in phospholipid-coated micro-meter scale closed spheres (MPDs). In a MPD, the mixture of T4 DNA and F-actin was found to phase-separate into F-actin phase (Px) and T4 DNA rich phase (P2), where the manner of the segregation is dependent on the total volume fraction of F-actin and T4 DNA. In addition, we found that, in a MPD, T4 DNA forms bundles in a crowded micro-space with F-actin even in the absence of multivalent cations.
UR - http://www.scopus.com/inward/record.url?scp=62449336412&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=62449336412&partnerID=8YFLogxK
U2 - 10.1109/MHS.2008.4752423
DO - 10.1109/MHS.2008.4752423
M3 - Conference contribution
AN - SCOPUS:62449336412
SN - 9781424429196
T3 - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008
SP - 60
EP - 64
BT - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"
T2 - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation"
Y2 - 6 November 2008 through 9 November 2008
ER -