TY - JOUR
T1 - Brefeldin A inhibits the targeting of cathepsin D and cathepsin H to lysosomes in rat hepatocytes
AU - Oda, Kimimitsu
AU - Nishimura, Yukio
N1 - Funding Information:
Y. Ikehara (Fukuoka University) and K. Kato for their encouragements during the course We also thank Dr. A. Takatsuki for the brefeldin A. This work was supported in part for ScientificResearch from the Ministry of and Culture, Japan.
PY - 1989/8/30
Y1 - 1989/8/30
N2 - Effect of brefeldin A on the transport of lysosomal acid hydrolases (cathepsins D and H) was investigated in primary cultured rat hepatocytes. Both cathepsins were synthesized as proenzymes and progressively converted to mature enzymes in the control cells. However, BFA strongly inhibited the appearance of the mature enzymes in the cells in a dose dependent manner, suggesting that transport of newly synthesized lysosomal enzymes from the endoplasmic reticulum to lysosomes is blocked by the drug. The inhibitory effect by brefeldin A was reversible. Upon recovery from brefeldin A-intoxication, procathepsin D was effectively targeted into lysosomes, whereas a substantial amount of procathepsin H was found to be missorted, resulting in its secretion into the culture medium.
AB - Effect of brefeldin A on the transport of lysosomal acid hydrolases (cathepsins D and H) was investigated in primary cultured rat hepatocytes. Both cathepsins were synthesized as proenzymes and progressively converted to mature enzymes in the control cells. However, BFA strongly inhibited the appearance of the mature enzymes in the cells in a dose dependent manner, suggesting that transport of newly synthesized lysosomal enzymes from the endoplasmic reticulum to lysosomes is blocked by the drug. The inhibitory effect by brefeldin A was reversible. Upon recovery from brefeldin A-intoxication, procathepsin D was effectively targeted into lysosomes, whereas a substantial amount of procathepsin H was found to be missorted, resulting in its secretion into the culture medium.
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U2 - 10.1016/0006-291X(89)92124-4
DO - 10.1016/0006-291X(89)92124-4
M3 - Article
C2 - 2775262
AN - SCOPUS:0024443485
SN - 0006-291X
VL - 163
SP - 220
EP - 225
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -