TY - JOUR
T1 - Breakthrough in purification of fossil pollen for dating of sediments by a new large-particle on-chip sorter
AU - Kasai, Y.
AU - Leipe, C.
AU - Saito, M.
AU - Kitagawa, H.
AU - Lauterbach, S.
AU - Brauer, A.
AU - Tarasov, P. E.
AU - Gosla, T.
AU - Arai, F.
AU - Sakuma, S.
N1 - Publisher Copyright:
© 2021 American Association for the Advancement of Science. All rights reserved.
PY - 2021/4/14
Y1 - 2021/4/14
N2 - Particle sorting is a fundamental method in various fields of medical and biological research. However, existing sorting applications are not capable for high-Throughput sorting of large-size (100 micrometers) particles. Here, we present a novel on-chip sorting method using traveling vortices generated by on-demand microjet flows, which locally exceed laminar flow condition, allowing for high-Throughput sorting (5 kilohertz) with a record-wide sorting area of 520 micrometers. Using an activation system based on fluorescence detection, the method successfully sorted 160-micrometer microbeads and purified fossil pollen (maximum dimension around 170 micrometers) from lake sediments. Radiocarbon dates of sorting-derived fossil pollen concentrates proved accurate, demonstrating the method's ability to enhance building chronologies for paleoenvironmental records from sedimentary archives. The method is capable to cover urgent needs for high-Throughput large-particle sorting in genomics, metabolomics, and regenerative medicine and opens up new opportunities for the use of pollen and other microfossils in geochronology, paleoecology, and paleoclimatology.
AB - Particle sorting is a fundamental method in various fields of medical and biological research. However, existing sorting applications are not capable for high-Throughput sorting of large-size (100 micrometers) particles. Here, we present a novel on-chip sorting method using traveling vortices generated by on-demand microjet flows, which locally exceed laminar flow condition, allowing for high-Throughput sorting (5 kilohertz) with a record-wide sorting area of 520 micrometers. Using an activation system based on fluorescence detection, the method successfully sorted 160-micrometer microbeads and purified fossil pollen (maximum dimension around 170 micrometers) from lake sediments. Radiocarbon dates of sorting-derived fossil pollen concentrates proved accurate, demonstrating the method's ability to enhance building chronologies for paleoenvironmental records from sedimentary archives. The method is capable to cover urgent needs for high-Throughput large-particle sorting in genomics, metabolomics, and regenerative medicine and opens up new opportunities for the use of pollen and other microfossils in geochronology, paleoecology, and paleoclimatology.
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U2 - 10.1126/sciadv.abe7327
DO - 10.1126/sciadv.abe7327
M3 - Article
C2 - 33853775
AN - SCOPUS:85104484943
SN - 2375-2548
VL - 7
JO - Science Advances
JF - Science Advances
IS - 16
M1 - eabe7327
ER -