TY - JOUR
T1 - Blood-Compatible Poly(2-methoxyethyl acrylate) Induces Blebbing-like Phenomenon and Promotes Viability of Tumor Cells in Serum-Free Medium
AU - Tsai, Meng Yu
AU - Aratsu, Fumihiro
AU - Sekida, Shogo
AU - Kobayashi, Shingo
AU - Tanaka, Masaru
N1 - Funding Information:
This work was supported by the funding program Progress 100 (World Premier International Researcher Invitation Program) of Kyushu University and JSPS KAKENHI Grants JP17H00408, JP17H02086, JP19K20697, and JP19H05720. This work was supported in part by “Dynamic Alliance for Open Innovation Bridging Human, Environment and Materials” from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT).
Publisher Copyright:
Copyright © 2020 American Chemical Society.
PY - 2020/4/20
Y1 - 2020/4/20
N2 - Circulating tumor cells (CTCs) are highly related to tumor metastasis and an effective technique of detecting/isolating CTCs has been expected to be established for tumor treatments. Previously, we reported that platelets cannot adhere but tumor cells can adhere on poly(2-methoxyethyl acrylate) (PMEA) substrate. In this study, we report that cell viability of human fibrosarcoma (HT-1080) cells was promoted on PMEA substrate in serum-free medium. The significant blebbing-like phenomenon and spontaneous formation of cell aggregation were observed on PMEA substrate. Moreover, cell viability was promoted by activation of Akt signaling pathway via N-cadherin-mediated cell-cell contact. These results suggest that not only capture efficiency and purity but also high cell viability of CTCs can be accomplished by using PMEA substrate. PMEA substrate can be a promising candidate for medical applications such as in vitro screening of anticancer drugs and is an excellent platform for studies and diagnoses of tumor migration and metastasis.
AB - Circulating tumor cells (CTCs) are highly related to tumor metastasis and an effective technique of detecting/isolating CTCs has been expected to be established for tumor treatments. Previously, we reported that platelets cannot adhere but tumor cells can adhere on poly(2-methoxyethyl acrylate) (PMEA) substrate. In this study, we report that cell viability of human fibrosarcoma (HT-1080) cells was promoted on PMEA substrate in serum-free medium. The significant blebbing-like phenomenon and spontaneous formation of cell aggregation were observed on PMEA substrate. Moreover, cell viability was promoted by activation of Akt signaling pathway via N-cadherin-mediated cell-cell contact. These results suggest that not only capture efficiency and purity but also high cell viability of CTCs can be accomplished by using PMEA substrate. PMEA substrate can be a promising candidate for medical applications such as in vitro screening of anticancer drugs and is an excellent platform for studies and diagnoses of tumor migration and metastasis.
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U2 - 10.1021/acsabm.9b00885
DO - 10.1021/acsabm.9b00885
M3 - Article
C2 - 35025308
AN - SCOPUS:85085103900
SN - 2576-6422
VL - 3
SP - 1858
EP - 1864
JO - ACS Applied Bio Materials
JF - ACS Applied Bio Materials
IS - 4
ER -