TY - JOUR
T1 - Bitter taste receptor T2R1 activities were compatible with behavioral sensitivity to bitterness in chickens
AU - Hirose, Nozomi
AU - Kawabata, Yuko
AU - Kawabata, Fuminori
AU - Nishimura, Shotaro
AU - Tabata, Shoji
N1 - Funding Information:
We thank Dr Takashi Ueda (Nagoya City University) for providing the Gα 16/gust44 plasmid. We appreciate the technical assistance of The Research Support Center, Research Center for Human Disease Modeling, Kyushu University Graduate School of Medical Sciences. This study was supported in part by a grant to F.K. from the Funds for the Development of Human Resources in Science and Technology, the Japan Science and Technology Agency ; a grant to F.K. from JSPS KAKENHI ( #26850207 ); and a grant to F.K. from Kyushu University Interdisciplinary Programs in Education and Projects in Research Development (#26703).
Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Clarification of the mechanism of the sense of taste in chickens will provide information useful for creating and improving new feedstuffs for chickens, because the character of the taste receptors in oral tissues affects feeding behavior in animals. In this study, we focused on the sensitivity to bitterness in chickens. We cloned one of the bitter taste receptors, T2R1, from the chicken palate, constructed several biosensor-cells expressing chicken T2R1 (cT2R1), and determined a highly sensitive biosensor of cT2R1 among them. By using Ca2+ imaging methods, we identified two agonists of cT2R1, dextromethorphan (Dex) and diphenidol (Dip). Dex was a new agonist of cT2R1 that was more potent than Dip. In a behavioral drinking study, the intake volumes of solutions of these compounds were significantly lower than that of water in chickens. These aversive concentrations were identical to the concentrations that could activate cT2R1 in a cell-based assay. These results suggest that the cT2R1 activities induced by these agonists are linked to behavioral sensitivity to bitterness in chickens.
AB - Clarification of the mechanism of the sense of taste in chickens will provide information useful for creating and improving new feedstuffs for chickens, because the character of the taste receptors in oral tissues affects feeding behavior in animals. In this study, we focused on the sensitivity to bitterness in chickens. We cloned one of the bitter taste receptors, T2R1, from the chicken palate, constructed several biosensor-cells expressing chicken T2R1 (cT2R1), and determined a highly sensitive biosensor of cT2R1 among them. By using Ca2+ imaging methods, we identified two agonists of cT2R1, dextromethorphan (Dex) and diphenidol (Dip). Dex was a new agonist of cT2R1 that was more potent than Dip. In a behavioral drinking study, the intake volumes of solutions of these compounds were significantly lower than that of water in chickens. These aversive concentrations were identical to the concentrations that could activate cT2R1 in a cell-based assay. These results suggest that the cT2R1 activities induced by these agonists are linked to behavioral sensitivity to bitterness in chickens.
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U2 - 10.1016/j.bbrc.2015.03.056
DO - 10.1016/j.bbrc.2015.03.056
M3 - Article
C2 - 25796330
AN - SCOPUS:84937764044
SN - 0006-291X
VL - 460
SP - 464
EP - 468
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -