TY - GEN
T1 - Biomimetic polymer nanoparticles embedding quantum dots
AU - Ishihara, Kazuhiko
AU - Goto, Yusuke
AU - Matsuno, Ryosuke
N1 - Funding Information:
The authors thank Prof. Tomohiro Konno, Prof. Madoka Takai, The University of Tokyo for their helpful discussion. This research was financially supported by a Grant-in-Aid for Scientific Research (B: 21300176) from the Ministry of Education, Culture, Sports, Science, and Technology, Japan.
PY - 2011
Y1 - 2011
N2 - To develop new functional fluorescence probe based on semiconductor nanoparticles, such as quantum dots (QD)s, we investigated polymer particle embedding QDs and covered with artificial cell membrane-biointerface. These nanoparticles were prepared by assembling 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer as a platform and biomolecules immobilized on the surface of the nanoparticles. The fluorescence property of QDs remained after embedding in the polymer nanoparticles. The MPC polymer surface showed high resistance to non-specific cellular uptake due to the phosphorylcholine groups in the side chain. On the other hand, when cell-penetration oligopeptide, octaarginine was immobilized on the surface, they could permeate the membrane of cells effectively and good fluorescence based on QDs could be observed. Cytotoxicity and inflammation reaction was not produced by these nanoparticles even after immobilization of octapeptide. In conclusion, we could obtain stable fluorescence polymer nanoparticles covered with artificial cell membrane, which are useful as an excellent bioimaging probe and as a novel evaluation tool for biomolecular function in the target cells.
AB - To develop new functional fluorescence probe based on semiconductor nanoparticles, such as quantum dots (QD)s, we investigated polymer particle embedding QDs and covered with artificial cell membrane-biointerface. These nanoparticles were prepared by assembling 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer as a platform and biomolecules immobilized on the surface of the nanoparticles. The fluorescence property of QDs remained after embedding in the polymer nanoparticles. The MPC polymer surface showed high resistance to non-specific cellular uptake due to the phosphorylcholine groups in the side chain. On the other hand, when cell-penetration oligopeptide, octaarginine was immobilized on the surface, they could permeate the membrane of cells effectively and good fluorescence based on QDs could be observed. Cytotoxicity and inflammation reaction was not produced by these nanoparticles even after immobilization of octapeptide. In conclusion, we could obtain stable fluorescence polymer nanoparticles covered with artificial cell membrane, which are useful as an excellent bioimaging probe and as a novel evaluation tool for biomolecular function in the target cells.
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U2 - 10.1557/opl.2011.1505
DO - 10.1557/opl.2011.1505
M3 - Conference contribution
AN - SCOPUS:84860210700
SN - 9781618395573
T3 - Materials Research Society Symposium Proceedings
SP - 12
EP - 17
BT - Biomimetic Engineering of Micro- and Nanoparticles
T2 - 2011 MRS Spring Meeting
Y2 - 25 April 2011 through 29 April 2011
ER -