Biologically active protein nanoarrays generated using parallel dip-pen nanolithography

Seung Woo Lee, Byung Keun Oh, Raymond G. Sanedrin, Khalid Salaita, Tsuyohiko Fujigaya, Chad A. Mirkin

Research output: Contribution to journalArticlepeer-review

128 Citations (Scopus)


The generation of biologically active protein nanoarrays using parallel dip-pen nanolithography (DPN), was investigated. To generate nanoscale arrays of amine-reactive dot features on a gold substrate, which was prepared using a 26-pen array was coated with NHSC11SH by immersing it in a 10mM NHSC11SH acetonitrile solution for 10 s. The DPN-generated NHSC 11SH dot arrays served as a template for the immobilization of protein A/G. Through the use of the affinity binding of the antibodies on protein A/G, biologically active antibody nanoarrays can e generated over macroscopic distances through parallel DPN, demonstrating the versatility of this approach for making similar antibody structures in a relatively high-throughput manner. The results show that researchers in nanoscience and biology will consider new ways of using such patterned interfaces to probe biological systems.

Original languageEnglish
Pages (from-to)1133-1136
Number of pages4
JournalAdvanced Materials
Issue number9
Publication statusPublished - May 2 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Materials Science
  • Mechanics of Materials
  • Mechanical Engineering


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