Biologically active human bone morphogenetic protein 4 fused to collagen-binding domain produced in silkworm-baculovirus expression system

Saki Imai; Zhiqing Li; Kazuhiro Iiyama; Yoshitaka Miyagawa; Masashi Toyoda; Akihiro Umezawa; Hiroaki Mon; Takahiro Kusakabe; Kaito Yoshimura; Jae Man Lee

doi:10.11416/jibs.82.2_039

Biologically active human bone morphogenetic protein 4 fused to collagen-binding domain produced in silkworm-baculovirus expression system

Saki Imai, Zhiqing Li, Kazuhiro Iiyama, Yoshitaka Miyagawa, Masashi Toyoda, Akihiro Umezawa, Hiroaki Mon, Takahiro Kusakabe, Kaito Yoshimura, Jae Man Lee

Agricultural Bioresource Sciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Human bone morphogenetic protein 4 (BMP4) fused to the collagen-binding domain (CBD) of fbronectin was constructed and expressed in a silkworm-baculovirus expression system. When recombinant BmNPV was injected into the silkworm larvae and harvested after approximately 4 days, 0.22 mg/ml (0.1 mg/larva) of recombinant CBD-BMP4 was secreted into the silkworm haemolymph. Interestingly, cultured silkworm cells infected with the same recombinant virus could not secrete the recombinant CBD-BMP4 into culture media. The purifed rCBD-BMP4 showed Smad- and MAPK-stimulating activities in human UET-13 cells.

Original language	English
Pages (from-to)	39-44
Number of pages	6
Journal	Journal of Insect Biotechnology and Sericology
Volume	82
Issue number	2
DOIs	https://doi.org/10.11416/jibs.82.2_039
Publication status	Published - Dec 12 2013

All Science Journal Classification (ASJC) codes

Biotechnology
Applied Microbiology and Biotechnology
Business, Management and Accounting(all)
Agricultural and Biological Sciences(all)
Insect Science
Industrial and Manufacturing Engineering

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title = "Biologically active human bone morphogenetic protein 4 fused to collagen-binding domain produced in silkworm-baculovirus expression system",

abstract = "Human bone morphogenetic protein 4 (BMP4) fused to the collagen-binding domain (CBD) of fbronectin was constructed and expressed in a silkworm-baculovirus expression system. When recombinant BmNPV was injected into the silkworm larvae and harvested after approximately 4 days, 0.22 mg/ml (0.1 mg/larva) of recombinant CBD-BMP4 was secreted into the silkworm haemolymph. Interestingly, cultured silkworm cells infected with the same recombinant virus could not secrete the recombinant CBD-BMP4 into culture media. The purifed rCBD-BMP4 showed Smad- and MAPK-stimulating activities in human UET-13 cells.",

author = "Saki Imai and Zhiqing Li and Kazuhiro Iiyama and Yoshitaka Miyagawa and Masashi Toyoda and Akihiro Umezawa and Hiroaki Mon and Takahiro Kusakabe and Kaito Yoshimura and Lee, {Jae Man}",

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doi = "10.11416/jibs.82.2_039",

language = "English",

volume = "82",

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journal = "Journal of Insect Biotechnology and Sericology",

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publisher = "Japanese Society of Sericultural Sciences",

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TY - JOUR

T1 - Biologically active human bone morphogenetic protein 4 fused to collagen-binding domain produced in silkworm-baculovirus expression system

AU - Imai, Saki

AU - Li, Zhiqing

AU - Iiyama, Kazuhiro

AU - Miyagawa, Yoshitaka

AU - Toyoda, Masashi

AU - Umezawa, Akihiro

AU - Mon, Hiroaki

AU - Kusakabe, Takahiro

AU - Yoshimura, Kaito

AU - Lee, Jae Man

PY - 2013/12/12

Y1 - 2013/12/12

N2 - Human bone morphogenetic protein 4 (BMP4) fused to the collagen-binding domain (CBD) of fbronectin was constructed and expressed in a silkworm-baculovirus expression system. When recombinant BmNPV was injected into the silkworm larvae and harvested after approximately 4 days, 0.22 mg/ml (0.1 mg/larva) of recombinant CBD-BMP4 was secreted into the silkworm haemolymph. Interestingly, cultured silkworm cells infected with the same recombinant virus could not secrete the recombinant CBD-BMP4 into culture media. The purifed rCBD-BMP4 showed Smad- and MAPK-stimulating activities in human UET-13 cells.

AB - Human bone morphogenetic protein 4 (BMP4) fused to the collagen-binding domain (CBD) of fbronectin was constructed and expressed in a silkworm-baculovirus expression system. When recombinant BmNPV was injected into the silkworm larvae and harvested after approximately 4 days, 0.22 mg/ml (0.1 mg/larva) of recombinant CBD-BMP4 was secreted into the silkworm haemolymph. Interestingly, cultured silkworm cells infected with the same recombinant virus could not secrete the recombinant CBD-BMP4 into culture media. The purifed rCBD-BMP4 showed Smad- and MAPK-stimulating activities in human UET-13 cells.

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SN - 1346-8073

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JO - Journal of Insect Biotechnology and Sericology

JF - Journal of Insect Biotechnology and Sericology

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ER -

Biologically active human bone morphogenetic protein 4 fused to collagen-binding domain produced in silkworm-baculovirus expression system

Abstract

All Science Journal Classification (ASJC) codes

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