Biodistribution of locally or systemically transplanted osteoblast-like cells

Y. T. Okabe, T. Kondo, K. Mishima, Y. Hayase, K. Kato, M. Mizuno, N. Ishiguro, H. Kitoh

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9 Citations (Scopus)


Objectives: In order to ensure safety of the cell-based therapy for bone regeneration, we examined in vivo biodistribution of locally or systemically transplanted osteoblast-like cells generated from bone marrow (BM) derived mononuclear cells. Methods: BM cells obtained from a total of 13 Sprague-Dawley (SD) green fluorescent protein transgenic (GFP-Tg) rats were culture-expanded in an osteogenic differentiation medium for three weeks. Osteoblast-like cells were then locally transplanted with collagen scaffolds to the rat model of segmental bone defect. Donor cells were also intravenously infused to the normal Sprague-Dawley (SD) rats for systemic biodistribution. The flow cytometric and histological analyses were performed for cellular tracking after transplantation. Results: Locally transplanted donor cells remained within the vicinity of the transplantation site without migrating to other organs. Systemically administered large amounts of osteoblast-like cells were cleared from various organ tissues within three days of transplantation and did not show any adverse effects in the transplanted rats. Conclusions: We demonstrated a precise assessment of donor cell biodistribution that further augments prospective utility of regenerative cell therapy.

Original languageEnglish
Pages (from-to)76-81
Number of pages6
JournalBone and Joint Research
Issue number3
Publication statusPublished - Mar 2014
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Surgery
  • Orthopedics and Sports Medicine


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