TY - JOUR
T1 - Biocompatibility of polyvinylalcohol gel as a vitreous substitute
AU - Maruoka, Shinji
AU - Matsuura, Toyoaki
AU - Kawasaki, Kensuke
AU - Okamoto, Masahiro
AU - Yoshiaki, Hara
AU - Kodama, Makoto
AU - Sugiyama, Masaaki
AU - Annaka, Masahiko
N1 - Funding Information:
The authors thank the late Dr. Aizo Yamauchi and Prof. Mototsugu Saishin for valuable discussions and suggestions. The work was partly supported by a Grant-in-Aid (No. 16591763 for T. M. and No. 17350060 for M. A.) from the Ministry of Education, Culture, Sports, Science, and Technology (for M. Annaka). M. A. and T. M. acknowledge the financial support from the Suzuken Memorial Foundation.
PY - 2006/8/1
Y1 - 2006/8/1
N2 - Polyvinylalcohol (PVA) hydrogel cross-linked by gamma irradiation was assessed as a possible vitreous substitute. From a series of experiments, rise of intraocular pressure and inflammatory changes in the vitreous cavity after operation were observed in some cases. Crab-eating macaques were used for this experiment. PVA gels were injected into vitreous cavity after vitrectomy and followed clinically by opthalmoscopy, tonometry, fundus photography, electroretinogram (ERG), chemotaxis, and flare cell meter. Histopathologic examination by light and electron microscopy was performed after 3 months. As a result, there were no significant changes in ophthalmoscopic findings. No abnormal rising of intraocular pressure (IOP) was recognized. ERG did not show meaningful amplitude weakness. From the photon counting of flare cell meter, significant break of blood-aqueous barrier and blood-retinal barrier was not observed. Histopathologic examination revealed that all layers of the retina were intact and no loss of tissue was evident. However, in PVA gel-injected eyes, some vacuolations of the inner retina were found in some specimens. To conclude, PVA gel was well tolerated in these experiments. The gel with a network similar to the vitreous body showed the best biocompatibility, though it is necessary to investigate the biocompatibility for the long-term. PVA gel is a good candidate for a vitreous substitute.
AB - Polyvinylalcohol (PVA) hydrogel cross-linked by gamma irradiation was assessed as a possible vitreous substitute. From a series of experiments, rise of intraocular pressure and inflammatory changes in the vitreous cavity after operation were observed in some cases. Crab-eating macaques were used for this experiment. PVA gels were injected into vitreous cavity after vitrectomy and followed clinically by opthalmoscopy, tonometry, fundus photography, electroretinogram (ERG), chemotaxis, and flare cell meter. Histopathologic examination by light and electron microscopy was performed after 3 months. As a result, there were no significant changes in ophthalmoscopic findings. No abnormal rising of intraocular pressure (IOP) was recognized. ERG did not show meaningful amplitude weakness. From the photon counting of flare cell meter, significant break of blood-aqueous barrier and blood-retinal barrier was not observed. Histopathologic examination revealed that all layers of the retina were intact and no loss of tissue was evident. However, in PVA gel-injected eyes, some vacuolations of the inner retina were found in some specimens. To conclude, PVA gel was well tolerated in these experiments. The gel with a network similar to the vitreous body showed the best biocompatibility, though it is necessary to investigate the biocompatibility for the long-term. PVA gel is a good candidate for a vitreous substitute.
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U2 - 10.1080/02713680600813854
DO - 10.1080/02713680600813854
M3 - Article
C2 - 16877268
AN - SCOPUS:33746821873
SN - 0271-3683
VL - 31
SP - 599
EP - 606
JO - Current Eye Research
JF - Current Eye Research
IS - 7-8
ER -