@article{7a63fa12832748138e8e0e17d3a777e4,
title = "Biochemical and structural characterization of a thermostable Dps protein with His-type ferroxidase centers and outer metal-binding sites",
abstract = "The DNA-binding protein from starved cells (Dps) is found in a wide range of microorganisms, and it has been well characterized. However, little is known about Dps proteins from nonheterocystous filamentous cyanobacteria. In this study, a Dps protein from the thermophilic nonheterocystous filamentous cyanobacterium Thermoleptolyngbya sp. O-77 (TlDps1) was purified and characterized. PAGE and CD analyses of TlDps1 demonstrated that it had higher thermostability than previously reported Dps proteins. X-ray crystallographic analysis revealed that TlDps1 possessed His-type ferroxidase centers within the cavity and unique metal-binding sites located on the surface of the protein, which presumably contributed to its exceedingly high thermostability.",
author = "Takuo Minato and Takamasa Teramoto and Yoshimitsu Kakuta and Seiji Ogo and Yoon, {Ki Seok}",
note = "Funding Information: We thank R. Matsuo (Malvern Panalytical) for his help with SEC‐RALS measurement and the staff members of the beamline BL45XU facilities at SPring‐8 for their help with data collection. This work was supported in part by JST CREST Grant Number JPMJCR18R2 (SO), JSPS KAKENHI Grant Numbers 18H02091 (KY) and 18J00191 (TM), and the World Premier International Research Center Initiative (WPI), Japan. TM was supported by the JSPS through a Research Fellowship for Young Scientists. Funding Information: We thank R. Matsuo (Malvern Panalytical) for his help with SEC-RALS measurement and the staff members of the beamline BL45XU facilities at SPring-8 for their help with data collection. This work was supported in part by JST CREST Grant Number JPMJCR18R2 (SO), JSPS KAKENHI Grant Numbers 18H02091 (KY) and 18J00191 (TM), and the World Premier International Research Center Initiative (WPI), Japan. TM was supported by the JSPS through a Research Fellowship for Young Scientists. Publisher Copyright: {\textcopyright} 2020 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.",
year = "2020",
month = jul,
day = "1",
doi = "10.1002/2211-5463.12837",
language = "English",
volume = "10",
pages = "1219--1229",
journal = "FEBS Open Bio",
issn = "2211-5463",
publisher = "Elsevier BV",
number = "7",
}