Biocatalysis in water-in-ionic liquid microemulsions: A case study with horseradish peroxidase

M. Moniruzzaman, N. Kamiya, M. Goto

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121 Citations (Scopus)


In this article we report the first results on the enzymatic activity of horseradish peroxidase (HRP) microencapsulated in water-in-ionic liquid (w/IL) microemulsions using pyrogallol as the substrate. Toward this goal, the system used in this study was composed of anionic surfactant AOT (sodium bis(2-ethyl-1-hexyl)sulfosuccinate)/hydrophobic IL [C8mim][Tf 2N] (1-octyl-3-methyl imidazolium bis(trifluoromethylsulfonyl)amide)/ water/l-hexanol. In this system, the catalytic activity of HRP was measured as a function of substrate concentrations, W0 (molar ratio of water to surfactant), pH, and 1-hexanol content. The curve of the activity-W0 profile was found to be hyperbolic for the new microemulsion. The apparent Michaelis-Menten kinetic parameters (Kcat and Km) were estimated and compared to those obtained from a conventional microemulsion. Apparently, it was found that HRP-catalyzed oxidation of pyrogallol by hydrogen peroxide in IL microemulsuions is much more effective than in a conventional AOT/water/isooctane microemulsion. The stability of HRP solubilized in the newly developed w/IL microemulsions was examined, and it was found that HRP retained almost 70% of its initial activity after incubation at 28 °C for 30 h.

Original languageEnglish
Pages (from-to)977-982
Number of pages6
Issue number2
Publication statusPublished - Jan 20 2009

All Science Journal Classification (ASJC) codes

  • General Materials Science
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry


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