TY - JOUR
T1 - Biobutanol production using high cell density fermentation in a large extractant volume
AU - Darmayanti, Rizki Fitria
AU - Tashiro, Yukihiro
AU - Sakai, Kenji
AU - Sanomoto, Kenji
AU - Susanti, Ari
AU - Palupi, Bekti
AU - Rizkiana, Meta Fitri
N1 - Funding Information:
Rizki Fitria Darmayanti acknowledges the Ministry of Educatio, nCulture, Spots,rSciece nandTechnology (MEXT), Japan for the fellowship. This work was partially suppotedrby JSPS KAKENHI Grant Number JP16K14890 and by Japan Sciencean d Technology Agency as part of the e-ASIA Joint ReserchaProgram (e-ASIA JRP). Thee isrnoco flicnts of inteetrtosdeclare.
Funding Information:
Rizki Fitria Darmayanti acknowledges the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan for the fellowship. This work was partially supported by JSPS KAKENHI Grant Number JP16K14890 and by Japan Science and Technology Agency as part of the e-ASIA Joint Research Program (e-ASIA JRP). There is no conflicts of interest to declare.
Publisher Copyright:
© 2020, Diponegoro university Indonesia - Center of Biomass and Renewable Energy (CBIORE). All rights reserved.
PY - 2020/10
Y1 - 2020/10
N2 - Biobutanol is well known as a suitable substitute for gasoline, which can be applied without engine modification. Butanol toxicity to the producer strain causes difficulties to grow strain of higher than 4 g/L dry cell weight and to produce butanol higher than 20 g/L. Fermentation using high initial cell density has been reported to enhance butanol productivity. In addition, oleyl alcohol has been recognized for effective extraction of butanol because of its selectivity and biocompatibility with reduced the effect of toxicity. Butanol fermentation with high cell density and large extractant volume has not been reported and is expected to improve butanol production in a minimum medium volume setting. Clostridium saccharoperbutylacetonicum N1-4, C. beijerinckii NCIMB 8052 (8052), and C. acetobutylicum ATCC 824 (824) were used in this study. Three kinds of media, TYA, TY, and TY-CaCO3, were used in this conventional extractive fermentation. Then, in situ extractive fermentation with Ve/Vb ratios at 0.1, 0.5, 1.0, and 10 were used. Total butanol concentration was defined as the broth-based total butanol, which is the total amount of butanol produced in broth and extractant per the volume of broth. TYA medium yielded the highest total butanol concentrations at N1-4 (12 g/L), 8052 (11 g/L), and 824 (15 g/L), and the highest partition coefficient (3.7) among the three media with similar Ve/Vb ratio at 0.5. N1-4 yielded the highest increment of total butanol production (22 g/L) in the extractive fermentation with high cell density. Low butanol concentration of 0.8 g/L in the broth was maintained using the extractant at a broth volume ratio (Ve/Vb) much lower than 4.4 g/L with a ratio of 0.5. Ve/Vb ratio of 10 which provided 2-fold higher total butanol concentration (28 g/L) than that of 11 g/L obtained using a Ve/Vb ratio of 0.5. These results indicated that a larger volume of extractant to broth improved total butanol concentration by reducing butanol toxicity and led to high medium based butanol yield in fermentation using high cell density.
AB - Biobutanol is well known as a suitable substitute for gasoline, which can be applied without engine modification. Butanol toxicity to the producer strain causes difficulties to grow strain of higher than 4 g/L dry cell weight and to produce butanol higher than 20 g/L. Fermentation using high initial cell density has been reported to enhance butanol productivity. In addition, oleyl alcohol has been recognized for effective extraction of butanol because of its selectivity and biocompatibility with reduced the effect of toxicity. Butanol fermentation with high cell density and large extractant volume has not been reported and is expected to improve butanol production in a minimum medium volume setting. Clostridium saccharoperbutylacetonicum N1-4, C. beijerinckii NCIMB 8052 (8052), and C. acetobutylicum ATCC 824 (824) were used in this study. Three kinds of media, TYA, TY, and TY-CaCO3, were used in this conventional extractive fermentation. Then, in situ extractive fermentation with Ve/Vb ratios at 0.1, 0.5, 1.0, and 10 were used. Total butanol concentration was defined as the broth-based total butanol, which is the total amount of butanol produced in broth and extractant per the volume of broth. TYA medium yielded the highest total butanol concentrations at N1-4 (12 g/L), 8052 (11 g/L), and 824 (15 g/L), and the highest partition coefficient (3.7) among the three media with similar Ve/Vb ratio at 0.5. N1-4 yielded the highest increment of total butanol production (22 g/L) in the extractive fermentation with high cell density. Low butanol concentration of 0.8 g/L in the broth was maintained using the extractant at a broth volume ratio (Ve/Vb) much lower than 4.4 g/L with a ratio of 0.5. Ve/Vb ratio of 10 which provided 2-fold higher total butanol concentration (28 g/L) than that of 11 g/L obtained using a Ve/Vb ratio of 0.5. These results indicated that a larger volume of extractant to broth improved total butanol concentration by reducing butanol toxicity and led to high medium based butanol yield in fermentation using high cell density.
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U2 - 10.14710/ijred.2020.29986
DO - 10.14710/ijred.2020.29986
M3 - Article
AN - SCOPUS:85089685742
SN - 2252-4940
VL - 9
SP - 431
EP - 437
JO - International Journal of Renewable Energy Development
JF - International Journal of Renewable Energy Development
IS - 3
ER -
