Bioactivities of volatile components from nepalese artemisia species

Prabodh Satyal, Prajwal Paudel, Ananad Kafle, Suraj K. Pokharel, Bimala Lamichhane, Noura S. Dosoky, Debra M. Moriarity, William N. Setzer

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)


The essential oils from the leaves of Artemisia dubia, A. indica, and A. vulgaris growing wild in Nepal were obtained by hydrodistillation and analyzed by GC-MS. The major components in A. dubia oil were chrysanthenone (29.0%), coumarin (18.3%), and camphor (16.4%). A. indica oil was dominated by ascaridole (15.4%), isoascaridole (9.9%), trans-p-mentha-2,8-dien-1-ol (9.7%), and trans-verbenol (8.4%). The essential oil of Nepalese A. vulgaris was rich in α-thujone (30.5%), 1,8-cineole (12.4%), and camphor (10.3%). The essential oils were screened for phytotoxic activity against Lactuca sativa (lettuce) and Lolium perenne (perennial ryegrass) using both seed germination and seedling growth, and all three Artemisia oils exhibited notable allelopathic activity. A. dubia oil showed in-vitro cytotoxic activity on MCF-7 cells (100% kill at 100 μg/mL) and was also marginally antifungal against Aspergillus niger (MIC = 313 μg/mL). DFT calculations (B3LYP/6-31G*) revealed thermal decomposition of ascaridole to be energetically accessible at hydrodistillation and GC conditions, but these are spin-forbidden processes. If decomposition does occur, it likely proceeds by way of homolytic peroxide bond cleavage rather than retro-Diels-Alder elimination of molecular oxygen.

Original languageEnglish
Pages (from-to)1651-1658
Number of pages8
JournalNatural product communications
Issue number12
Publication statusPublished - Dec 2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Drug Discovery
  • Complementary and alternative medicine
  • Plant Science
  • Pharmacology


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