Binding of lectins to DNA micro-assemblies: Modification of nucleo-cages with lactose-conjugated psoralen

Kwonil Kim, Kazunori Matsuura, Nobuo Kimizuka

    Research output: Contribution to journalArticlepeer-review

    24 Citations (Scopus)

    Abstract

    Spherical DNA micro-assemblies appended with lactose units (Lactose-nucleo-cages, Lac-NC) are newly developed. DNA spherical assemblies self-assembled from suitably designed three oligodeoxyribonucleotides (ODNs) 1-3 were cross-linked by lactose-conjugated psoralen derivative 4. Confocal laser scanning fluorescence microscopy (CLSM) observation of Lac-NC shows that rhodamine labeled peanut lectin (Rho-PNA: a galactose-specific lectin) binds to lactose-modified nucleo-cages with higher affinity compared to that of unmodified nucleo-cages. Binding isotherm experiments indicated that the apparent affinity constant of Rho-PNA to Lac-NC was in the order of 105 M-1.

    Original languageEnglish
    Pages (from-to)4311-4317
    Number of pages7
    JournalBioorganic and Medicinal Chemistry
    Volume15
    Issue number12
    DOIs
    Publication statusPublished - Jun 15 2007

    All Science Journal Classification (ASJC) codes

    • Biochemistry
    • Molecular Medicine
    • Molecular Biology
    • Pharmaceutical Science
    • Drug Discovery
    • Clinical Biochemistry
    • Organic Chemistry

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