Bessel-beam illumination Raman microscopy

Kazuki Bando, Shumpei Yabuuchi, Menglu Li, Toshiki Kubo, Ryosuke Oketani, Nicholas I. Smith, Katsumasa Fujita

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


We demonstrate the use of Bessel beams for side illumination slit-scanning Raman imaging for label-free and hyperspectral analysis of cell spheroids. The background elimination by the side illumination and the aberration-resistant Bessel beam drastically improves the image contrast in Raman observation, allowing label-free investigation of intracellular molecules in thick biological samples. Live cell spheroids were observed to confirm the improvement in image contrast and background reduction with Bessel illumination compared to conventional epi-line illumination.

Original languageEnglish
Pages (from-to)3161-3170
Number of pages10
JournalBiomedical Optics Express
Issue number6
Publication statusPublished - Jun 1 2022
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics


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