TY - JOUR
T1 - Base structure consisting of an endothelialized vascular-tree network and hepatocytes for whole liver engineering
AU - Shirakigawa, Nana
AU - Takei, Takayuki
AU - Ijima, Hiroyuki
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research (B): 22360348 from the Ministry of Education, Culture, Sports, Science and Technology of Japan and Grant Number 244676 from Japan Society for the Promotion of Science.
PY - 2013/12
Y1 - 2013/12
N2 - Reconstructed liver has been desired as a liver substitute for transplantation. However, reconstruction of a whole liver has not been achieved because construction of a vascular network at an organ scale is very difficult. We focused on decellularized liver (DC-liver) as an artificial scaffold for the construction of a hierarchical vascular network. In this study, we obtained DC-liver and the tubular network structure in which both portal vein and hepatic vein systems remained intact. Furthermore, endothelialization of the tubular structure in DC-liver was achieved, which prevented blood leakage from the tubular structure. In addition, hepatocytes suspended in a collagen sol were injected from the surroundings using a syringe as a suitable procedure for liver cell inoculation. In summary, we developed a base structure consisting of an endothelialized vascular-tree network and hepatocytes for whole liver engineering.
AB - Reconstructed liver has been desired as a liver substitute for transplantation. However, reconstruction of a whole liver has not been achieved because construction of a vascular network at an organ scale is very difficult. We focused on decellularized liver (DC-liver) as an artificial scaffold for the construction of a hierarchical vascular network. In this study, we obtained DC-liver and the tubular network structure in which both portal vein and hepatic vein systems remained intact. Furthermore, endothelialization of the tubular structure in DC-liver was achieved, which prevented blood leakage from the tubular structure. In addition, hepatocytes suspended in a collagen sol were injected from the surroundings using a syringe as a suitable procedure for liver cell inoculation. In summary, we developed a base structure consisting of an endothelialized vascular-tree network and hepatocytes for whole liver engineering.
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U2 - 10.1016/j.jbiosc.2013.05.020
DO - 10.1016/j.jbiosc.2013.05.020
M3 - Article
C2 - 23770123
AN - SCOPUS:84886385852
SN - 1389-1723
VL - 116
SP - 740
EP - 745
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 6
ER -