TY - JOUR
T1 - Bacteriostatic activity of whey acidic protein (WAP)
AU - Iwamori, Tokuko
AU - Nukumi, Naoko
AU - Itoh, Kikuji
AU - Kano, Kiyoshi
AU - Naito, Kunihiko
AU - Kurohmaru, Masamichi
AU - Yamanouchi, Keitaro
AU - Tojo, Hideaki
N1 - Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2010
Y1 - 2010
N2 - We have previously reported the action of whey acidic protein (WAP) inhibiting the proliferation of mouse mammary epithelial cells in the experiments utilizing in vivo and in vitro systems. We report herein the bacteriostatic activity of WAP. Western blot analysis demonstrated successful isolation of WAP from whey fractions of rat milk by column chromatography. The WAP fraction inhibited the growth of Staphylococcus aureus JCM2413 in a dose-dependent manner, but did not inhibit the growth of Escherichia coli. The bacteriostatic activity of WAP was highest at pH 6.6 and was not affected by the presence of 150 mM NaCl. A scanning electron micrograph of bacteria treated with WAP exhibited the disruption of the bacterial cell walls.
AB - We have previously reported the action of whey acidic protein (WAP) inhibiting the proliferation of mouse mammary epithelial cells in the experiments utilizing in vivo and in vitro systems. We report herein the bacteriostatic activity of WAP. Western blot analysis demonstrated successful isolation of WAP from whey fractions of rat milk by column chromatography. The WAP fraction inhibited the growth of Staphylococcus aureus JCM2413 in a dose-dependent manner, but did not inhibit the growth of Escherichia coli. The bacteriostatic activity of WAP was highest at pH 6.6 and was not affected by the presence of 150 mM NaCl. A scanning electron micrograph of bacteria treated with WAP exhibited the disruption of the bacterial cell walls.
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U2 - 10.1292/jvms.08-0331
DO - 10.1292/jvms.08-0331
M3 - Article
C2 - 20009425
AN - SCOPUS:77953750929
SN - 0916-7250
VL - 72
SP - 621
EP - 625
JO - Journal of Veterinary Medical Science
JF - Journal of Veterinary Medical Science
IS - 5
ER -