Bacteriostatic activity of whey acidic protein (WAP)

Tokuko Iwamori, Naoko Nukumi, Kikuji Itoh, Kiyoshi Kano, Kunihiko Naito, Masamichi Kurohmaru, Keitaro Yamanouchi, Hideaki Tojo

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


We have previously reported the action of whey acidic protein (WAP) inhibiting the proliferation of mouse mammary epithelial cells in the experiments utilizing in vivo and in vitro systems. We report herein the bacteriostatic activity of WAP. Western blot analysis demonstrated successful isolation of WAP from whey fractions of rat milk by column chromatography. The WAP fraction inhibited the growth of Staphylococcus aureus JCM2413 in a dose-dependent manner, but did not inhibit the growth of Escherichia coli. The bacteriostatic activity of WAP was highest at pH 6.6 and was not affected by the presence of 150 mM NaCl. A scanning electron micrograph of bacteria treated with WAP exhibited the disruption of the bacterial cell walls.

Original languageEnglish
Pages (from-to)621-625
Number of pages5
JournalJournal of Veterinary Medical Science
Issue number5
Publication statusPublished - 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • veterinary(all)


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