Bacteria-laden microgels as autonomous three-dimensional environments for stem cell engineering

K. Witte; A. Rodrigo-Navarro; M. Salmeron-Sanchez

doi:10.1016/j.mtbio.2019.100011

Bacteria-laden microgels as autonomous three-dimensional environments for stem cell engineering

K. Witte, A. Rodrigo-Navarro, M. Salmeron-Sanchez

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

A one-step microfluidic system is developed in this study which enables the encapsulation of stem cells and genetically engineered non-pathogenic bacteria into a so-called three-dimensional (3D) pearl lace–like microgel of alginate with high level of monodispersity and cell viability. The alginate-based microgel constitutes living materials that control stem cell differentiation in either an autonomous or heteronomous manner. The bacteria (Lactococcus lactis) encapsulated within the construct surface display adhesion fragments (III_7-10 fragment of human fibronectin) for integrin binding while secreting growth factors (recombinant human bone morphogenetic protein-2) to induce osteogenic differentiation of human bone marrow–derived mesenchymal stem cells. We concentrate on interlinked pearl lace microgels that enabled us to prototype a low-cost 3D bioprinting platform with highly tunable properties.

Original language	English
Article number	100011
Journal	Materials Today Bio
Volume	2
DOIs	https://doi.org/10.1016/j.mtbio.2019.100011
Publication status	Published - Mar 2019
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biomaterials
Biomedical Engineering
Molecular Biology
Cell Biology

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10.1016/j.mtbio.2019.100011

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title = "Bacteria-laden microgels as autonomous three-dimensional environments for stem cell engineering",

abstract = "A one-step microfluidic system is developed in this study which enables the encapsulation of stem cells and genetically engineered non-pathogenic bacteria into a so-called three-dimensional (3D) pearl lace–like microgel of alginate with high level of monodispersity and cell viability. The alginate-based microgel constitutes living materials that control stem cell differentiation in either an autonomous or heteronomous manner. The bacteria (Lactococcus lactis) encapsulated within the construct surface display adhesion fragments (III7-10 fragment of human fibronectin) for integrin binding while secreting growth factors (recombinant human bone morphogenetic protein-2) to induce osteogenic differentiation of human bone marrow–derived mesenchymal stem cells. We concentrate on interlinked pearl lace microgels that enabled us to prototype a low-cost 3D bioprinting platform with highly tunable properties.",

author = "K. Witte and A. Rodrigo-Navarro and M. Salmeron-Sanchez",

note = "Funding Information: The support from the UK Engineering and Physical Sciences Research Council ( EP/P001114/1 ) is acknowledged. Publisher Copyright: {\textcopyright} 2019 The Authors",

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AU - Witte, K.

AU - Rodrigo-Navarro, A.

AU - Salmeron-Sanchez, M.

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AB - A one-step microfluidic system is developed in this study which enables the encapsulation of stem cells and genetically engineered non-pathogenic bacteria into a so-called three-dimensional (3D) pearl lace–like microgel of alginate with high level of monodispersity and cell viability. The alginate-based microgel constitutes living materials that control stem cell differentiation in either an autonomous or heteronomous manner. The bacteria (Lactococcus lactis) encapsulated within the construct surface display adhesion fragments (III7-10 fragment of human fibronectin) for integrin binding while secreting growth factors (recombinant human bone morphogenetic protein-2) to induce osteogenic differentiation of human bone marrow–derived mesenchymal stem cells. We concentrate on interlinked pearl lace microgels that enabled us to prototype a low-cost 3D bioprinting platform with highly tunable properties.

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Bacteria-laden microgels as autonomous three-dimensional environments for stem cell engineering

Abstract

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