TY - JOUR
T1 - Bacteria-Based Materials for Stem Cell Engineering
AU - Hay, Jake J.
AU - Rodrigo-Navarro, Aleixandre
AU - Petaroudi, Michaela
AU - Bryksin, Anton V.
AU - García, Andrés J.
AU - Barker, Thomas H.
AU - Dalby, Matthew J.
AU - Salmeron-Sanchez, Manuel
N1 - Funding Information:
J.J.H. and A.R.-N. contributed equally to this work. The support from the European Research Council (ERC306990), the UK Engineering and Physical Sciences Research Council (EP/P001114/1), and the Leverhulme Trust (RPG-2015-191) is acknowledged. M.J.D. and M.S.-S. designed the concept. A.R.-N., A.V.B., A.J.G., T.H.B., M.J.D., and M.S.-S. designed the experiments. J.J.H., A.R.-N., and M.P. performed the experiments. J.J.H. and A.R.-N. prepared figures. J.J.H., A.R.-N., A.J.G., T.H.B., M.J.D., and M.S.-S. wrote the paper. The hMSCs used were purchased from PromoCell.
Publisher Copyright:
© 2018 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2018/10/25
Y1 - 2018/10/25
N2 - Materials can be engineered to deliver specific biological cues that control stem cell growth and differentiation. However, current materials are still limited for stem cell engineering as stem cells are regulated by a complex biological milieu that requires spatiotemporal control. Here a new approach of using materials that incorporate designed bacteria as units that can be engineered to control human mesenchymal stem cells (hMSCs), in a highly dynamic-temporal manner, is presented. Engineered Lactococcus lactis spontaneously colonizes a variety of material surfaces (e.g., polymers, metals, and ceramics) and is able to maintain growth and induce differentiation of hMSCs in 2D/3D surfaces and hydrogels. Controlled, dynamic, expression of fibronectin fragments supports stem cell growth, whereas inducible-temporal regulation of secreted bone morphogenetic protein-2 drives osteogenesis in an on-demand manner. This approach enables stem cell technologies using material systems that host symbiotic interactions between eukaryotic and prokaryotic cells.
AB - Materials can be engineered to deliver specific biological cues that control stem cell growth and differentiation. However, current materials are still limited for stem cell engineering as stem cells are regulated by a complex biological milieu that requires spatiotemporal control. Here a new approach of using materials that incorporate designed bacteria as units that can be engineered to control human mesenchymal stem cells (hMSCs), in a highly dynamic-temporal manner, is presented. Engineered Lactococcus lactis spontaneously colonizes a variety of material surfaces (e.g., polymers, metals, and ceramics) and is able to maintain growth and induce differentiation of hMSCs in 2D/3D surfaces and hydrogels. Controlled, dynamic, expression of fibronectin fragments supports stem cell growth, whereas inducible-temporal regulation of secreted bone morphogenetic protein-2 drives osteogenesis in an on-demand manner. This approach enables stem cell technologies using material systems that host symbiotic interactions between eukaryotic and prokaryotic cells.
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U2 - 10.1002/adma.201804310
DO - 10.1002/adma.201804310
M3 - Article
C2 - 30209838
AN - SCOPUS:85053387014
SN - 0935-9648
VL - 30
JO - Advanced Materials
JF - Advanced Materials
IS - 43
M1 - 1804310
ER -