Backward multiplex coherent anti-Stokes Raman (CARS) spectroscopic imaging with electron-multiplying CCD (EM-CCD) camera

Yusuke Murakami; Minami Yoshimura; W. J.Niels Klement; Atsuki Oda; Ryo Sakamoto; Miho Yakabe; Atsushi Matsumoto; Ryosuke Oketani; Philippe Leproux; Junichi Ikenouchi; Wesley R. Browne; Hideaki Kano

doi:10.1364/OPTCON.497869

Backward multiplex coherent anti-Stokes Raman (CARS) spectroscopic imaging with electron-multiplying CCD (EM-CCD) camera

Yusuke Murakami, Minami Yoshimura, W. J.Niels Klement, Atsuki Oda, Ryo Sakamoto, Miho Yakabe, Atsushi Matsumoto, Ryosuke Oketani, Philippe Leproux, Junichi Ikenouchi, Wesley R. Browne, Hideaki Kano

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

A multiplex CARS imaging system, equipped with an EM-CCD camera, was developed to improve the sensitivity of backward CARS imaging in biological analysis using an inverted microscope. The signal-to-noise ratio was improved by a factor of ca. 3 compared to a conventional CCD mode through the use of EM gain. When imaging epithelial cells in the backward CARS configuration, intracellular organelles such as lipid droplets and nuclei were spectroscopically identified with an exposure time of only 100 ms/pixel.

Original language	English
Pages (from-to)	2044-2054
Number of pages	11
Journal	Optics Continuum
Volume	2
Issue number	9
DOIs	https://doi.org/10.1364/OPTCON.497869
Publication status	Published - 2023

All Science Journal Classification (ASJC) codes

Electrical and Electronic Engineering
Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics

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10.1364/OPTCON.497869

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title = "Backward multiplex coherent anti-Stokes Raman (CARS) spectroscopic imaging with electron-multiplying CCD (EM-CCD) camera",

abstract = "A multiplex CARS imaging system, equipped with an EM-CCD camera, was developed to improve the sensitivity of backward CARS imaging in biological analysis using an inverted microscope. The signal-to-noise ratio was improved by a factor of ca. 3 compared to a conventional CCD mode through the use of EM gain. When imaging epithelial cells in the backward CARS configuration, intracellular organelles such as lipid droplets and nuclei were spectroscopically identified with an exposure time of only 100 ms/pixel.",

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year = "2023",

doi = "10.1364/OPTCON.497869",

language = "English",

volume = "2",

pages = "2044--2054",

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T1 - Backward multiplex coherent anti-Stokes Raman (CARS) spectroscopic imaging with electron-multiplying CCD (EM-CCD) camera

AU - Murakami, Yusuke

AU - Yoshimura, Minami

AU - Klement, W. J.Niels

AU - Oda, Atsuki

AU - Sakamoto, Ryo

AU - Yakabe, Miho

AU - Matsumoto, Atsushi

AU - Oketani, Ryosuke

AU - Leproux, Philippe

AU - Ikenouchi, Junichi

AU - Browne, Wesley R.

AU - Kano, Hideaki

PY - 2023

Y1 - 2023

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AB - A multiplex CARS imaging system, equipped with an EM-CCD camera, was developed to improve the sensitivity of backward CARS imaging in biological analysis using an inverted microscope. The signal-to-noise ratio was improved by a factor of ca. 3 compared to a conventional CCD mode through the use of EM gain. When imaging epithelial cells in the backward CARS configuration, intracellular organelles such as lipid droplets and nuclei were spectroscopically identified with an exposure time of only 100 ms/pixel.

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JF - Optics Continuum

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Backward multiplex coherent anti-Stokes Raman (CARS) spectroscopic imaging with electron-multiplying CCD (EM-CCD) camera

Abstract

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