TY - JOUR
T1 - Bacillus subtilis YabA is involved in determining the timing and synchrony of replication initiation
AU - Hayashi, Miho
AU - Ogura, Yoshitoshi
AU - Harry, Elizabeth J.
AU - Ogasawara, Naotake
AU - Moriya, Shigeki
N1 - Funding Information:
We thank R.Y. Tsien, Howard Hughes Medical Institute at University of California, San Diego, for providing us a plasmid encoding Cfp variant W7. We also thank A.D. Grossman and L. Thompson for the gift of a dnaX-gfpmut2 plasmid and for useful comments, respectively. We are grateful to S. Samman and P. Petocz for statistical test of our data. This work was supported by grants-in-aid for scientific research (B) from Japan Society for the Promotion of Science to S.M. and N.O. and by a grant from the Australian Research Council to E.J.H. who is the recipient of an ARC QEII Research Fellowship.
PY - 2005/6/1
Y1 - 2005/6/1
N2 - It is shown here by flow cytometry that Bacillus subtilis YabA negatively regulates the timing of replication initiation. When the level of YabA was reduced, replication began at a decreased cell mass and when the level was increased, initiation was delayed. Synchrony of replication initiation was also disrupted at low levels of YabA. Yfp-YabA localized as foci in cells. Since YabA was reported to interact with DnaN (β subunit of DNA polymerase III), co-localization of Yfp-YabA with the polymerase was examined using a Cfp fusion with DnaX (τ subunit of DNA polymerase III). It is reported that YabA appears to localize at the replication forks only at a late stage of DNA replication.
AB - It is shown here by flow cytometry that Bacillus subtilis YabA negatively regulates the timing of replication initiation. When the level of YabA was reduced, replication began at a decreased cell mass and when the level was increased, initiation was delayed. Synchrony of replication initiation was also disrupted at low levels of YabA. Yfp-YabA localized as foci in cells. Since YabA was reported to interact with DnaN (β subunit of DNA polymerase III), co-localization of Yfp-YabA with the polymerase was examined using a Cfp fusion with DnaX (τ subunit of DNA polymerase III). It is reported that YabA appears to localize at the replication forks only at a late stage of DNA replication.
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U2 - 10.1016/j.femsle.2005.04.028
DO - 10.1016/j.femsle.2005.04.028
M3 - Article
C2 - 15927750
AN - SCOPUS:20344367271
SN - 0378-1097
VL - 247
SP - 73
EP - 79
JO - FEMS microbiology letters
JF - FEMS microbiology letters
IS - 1
ER -