TY - JOUR
T1 - B7-DC induced by IL-13 works as a feedback regulator in the effector phase of allergic asthma
AU - Matsumoto, Koichiro
AU - Fukuyama, Satoru
AU - Eguchi-Tsuda, Miyuki
AU - Nakano, Takako
AU - Matsumoto, Takafumi
AU - Matsumura, Mikiko
AU - Moriwaki, Atsushi
AU - Kan-o, Keiko
AU - Wada, Yoshihiro
AU - Yagita, Hideo
AU - Shin, Tahiro
AU - Pardoll, Drew M.
AU - Patcharee, Rirprajak
AU - Azuma, Miyuki
AU - Nakanishi, Yoichi
AU - Inoue, Hiromasa
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan. The authors thank Mses. Yuki Yoshioka, Tomoko Yoshimura, and Ayako Hashizume for technical assistance.
PY - 2008/1/4
Y1 - 2008/1/4
N2 - B7-DC is a costimulatory molecule belonging to the B7 family. We previously found that treatment with anti-B7-DC mAb during the effector phase enhances asthma phenotypes in mice. We investigated the mechanisms of B7-DC induction and how B7-DC regulates asthma phenotypes. In allergen-challenged IFN-γ-deficient mice, anti-B7-DC mAb failed to enhance the asthma phenotypes although the induction of B7-DC on dendritic cells of the mice was comparable with that on dendritic cells of wild-type mice. B7-DC on dendritic cells was up-regulated by IL-13 in vitro. The induction of B7-DC on dendritic cells after allergen challenge was attenuated by blockade of IL-13 in vivo. The asthma phenotypes were enhanced in B7-DC-deficient mice, more than in wild-type mice. The enhancement was concurrent with the down-regulation of IFN-γ and up-regulation of IL-13. These results suggest that B7-DC induced by IL-13 works as a feedback regulator by up-regulating IFN-γ production during the effector phase of allergic asthma.
AB - B7-DC is a costimulatory molecule belonging to the B7 family. We previously found that treatment with anti-B7-DC mAb during the effector phase enhances asthma phenotypes in mice. We investigated the mechanisms of B7-DC induction and how B7-DC regulates asthma phenotypes. In allergen-challenged IFN-γ-deficient mice, anti-B7-DC mAb failed to enhance the asthma phenotypes although the induction of B7-DC on dendritic cells of the mice was comparable with that on dendritic cells of wild-type mice. B7-DC on dendritic cells was up-regulated by IL-13 in vitro. The induction of B7-DC on dendritic cells after allergen challenge was attenuated by blockade of IL-13 in vivo. The asthma phenotypes were enhanced in B7-DC-deficient mice, more than in wild-type mice. The enhancement was concurrent with the down-regulation of IFN-γ and up-regulation of IL-13. These results suggest that B7-DC induced by IL-13 works as a feedback regulator by up-regulating IFN-γ production during the effector phase of allergic asthma.
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U2 - 10.1016/j.bbrc.2007.10.156
DO - 10.1016/j.bbrc.2007.10.156
M3 - Article
C2 - 17981145
AN - SCOPUS:36049010929
SN - 0006-291X
VL - 365
SP - 170
EP - 175
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -