Automated spectroscopic imaging of oxygen saturation in human retinal vessels

D. Nakamura, S. Sueda, N. Matsuoka, Y. Yoshinaga, H. Enaida, T. Okada, T. Ishibashi

Research output: Contribution to journalConference articlepeer-review

3 Citations (Scopus)


A new automatic visualization procedure for the oxygen saturation imaging from multi-spectral imaging of human retinal vessels has been proposed. Two-wavelength retinal fundus images at 545 and 560 nm, which were oxygen insensitive and oxygen sensitive, respectively, were captured by CCD cameras simultaneously through a beam splitter and interference filters. We applied a morphological processing technique to presume a distribution of incident light including the vessel parts and an optical density (OD) image of each wavelength image. And the OD ratio (OD560/OD545) image was calculated as a relative indicator of oxygen saturation. Furthermore, processing of line convergence index filter was adopted to identify the retinal vessels. Clear difference between retinal arteries and veins was observed in the automated imaging method. In addition, the decrease of oxygen saturation in the retinal artery without breathing could be monitored by the ODR. This method is possible to be applied to real-time monitoring for oxygen saturation of retinal vessels.

Original languageEnglish
Article number71631N
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Publication statusPublished - 2009
EventOphthalmic Technologies XIX - San Jose, CA, United States
Duration: Jan 24 2009Jan 26 2009

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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