TY - JOUR
T1 - Automated chemiluminescence immunoassay for a nonionic surfactant using a recycled spinning-pausing controlled washing procedure on a compact disc-type microfluidic platform
AU - Guo, Shuai
AU - Ishimatsu, Ryoichi
AU - Nakano, Koji
AU - Imato, Toshihiko
N1 - Funding Information:
The authors wish to acknowledge financial supports from the Global COE Program “Science for Future Molecular Systems” from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan and from the Japan Society of Promotion of the Science (JSPS, Grant no. 25288065 ).
Publisher Copyright:
© 2014 Elsevier B.V. All rights reserved.
PY - 2015/2
Y1 - 2015/2
N2 - A fully automated and integrated chemiluminescence immunoassay, carried out on a compact disc (CD)-type microfluidic platform, for the detection of alkylphenol polyethoxylates (APnEOs) is described. The pattern of the CD-type microchip was designed so as to permit the sequential solution delivery of the sample solution, the washing solution and the luminol solution, which are required in the chemiluminescence immunoassay process, along with a designed rotation program for spinning the CD-type microchip. The procedure for flowing the washing solution, the volume of which was limited on the CD-type microchip, was optimized by using a recycled spinning-pausing rotation program to overcome the non-specific adsorption of the horseradish peroxidase labeled APnEOs at the detection area. The detection limit of the immunoassay is about 10 ppb.
AB - A fully automated and integrated chemiluminescence immunoassay, carried out on a compact disc (CD)-type microfluidic platform, for the detection of alkylphenol polyethoxylates (APnEOs) is described. The pattern of the CD-type microchip was designed so as to permit the sequential solution delivery of the sample solution, the washing solution and the luminol solution, which are required in the chemiluminescence immunoassay process, along with a designed rotation program for spinning the CD-type microchip. The procedure for flowing the washing solution, the volume of which was limited on the CD-type microchip, was optimized by using a recycled spinning-pausing rotation program to overcome the non-specific adsorption of the horseradish peroxidase labeled APnEOs at the detection area. The detection limit of the immunoassay is about 10 ppb.
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U2 - 10.1016/j.talanta.2014.06.075
DO - 10.1016/j.talanta.2014.06.075
M3 - Article
C2 - 25435234
AN - SCOPUS:84913596410
SN - 0039-9140
VL - 133
SP - 100
EP - 106
JO - Talanta
JF - Talanta
ER -