TY - JOUR
T1 - Author Correction
T2 - Mechanisms of lncRNA biogenesis as revealed by nascent transcriptomics (Nature Reviews Molecular Cell Biology, (2022), 23, 6, (389-406), 10.1038/s41580-021-00447-6)
AU - Nojima, Takayuki
AU - Proudfoot, Nick J.
N1 - Publisher Copyright:
© 2022, Springer Nature Limited.
PY - 2022/12
Y1 - 2022/12
N2 - In the version of this article initially published, the technique fastGRO was presented in Fig. 2 and the main text as a chromatin- based run- on technique when in fact it is a nuclear run- on technique. Accordingly, in Fig. 2, right- hand “Techniques” section, the third balloon has been edited to include “fastGRO” and the seventh balloon edited to remove “fastGRO,” and the eighth sentence of the Fig. 2 legend has been amended to include fastGRO, as follows: “Nucleus: transcription run- on (TRO) analyses are performed in biochemically isolated nuclei using 5-bromouridine 5′-triphosphate (BrUTP) in GRO-seq37, 4- thiouridine (4sU) in fastGRO40 or biotin- labelled NTPs (biotin- NTP) in PRO- seq38.” Further, the penultimate sentence of the “In vitro RNA labelling” section has been amended to read “Finally, a variation on genomic TRO called fastGRO also employs 4- thiouridine (4sU) in the NRO reaction” (from the original “Finally, a variation on genomic TRO called fastGRO also employs the use of chromatin fractions as starting material but the nascent transcript is tagged with 4- thiouridine (4sU)….”). The changes are reflected in the HTML and PDF versions of the article.
AB - In the version of this article initially published, the technique fastGRO was presented in Fig. 2 and the main text as a chromatin- based run- on technique when in fact it is a nuclear run- on technique. Accordingly, in Fig. 2, right- hand “Techniques” section, the third balloon has been edited to include “fastGRO” and the seventh balloon edited to remove “fastGRO,” and the eighth sentence of the Fig. 2 legend has been amended to include fastGRO, as follows: “Nucleus: transcription run- on (TRO) analyses are performed in biochemically isolated nuclei using 5-bromouridine 5′-triphosphate (BrUTP) in GRO-seq37, 4- thiouridine (4sU) in fastGRO40 or biotin- labelled NTPs (biotin- NTP) in PRO- seq38.” Further, the penultimate sentence of the “In vitro RNA labelling” section has been amended to read “Finally, a variation on genomic TRO called fastGRO also employs 4- thiouridine (4sU) in the NRO reaction” (from the original “Finally, a variation on genomic TRO called fastGRO also employs the use of chromatin fractions as starting material but the nascent transcript is tagged with 4- thiouridine (4sU)….”). The changes are reflected in the HTML and PDF versions of the article.
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U2 - 10.1038/s41580-022-00551-1
DO - 10.1038/s41580-022-00551-1
M3 - Comment/debate
C2 - 36229539
AN - SCOPUS:85139760201
SN - 1471-0072
VL - 23
SP - 853
JO - Nature Reviews Molecular Cell Biology
JF - Nature Reviews Molecular Cell Biology
IS - 12
ER -