P450cl7 is the single enzyme mediating both 17α-hydroxylase and 17,20 lyase activities. We identified several human P450cl7 cDNA clones in a human adrenal cDNA library we constructed in XgtlO. A short clone containing the 3’-terminal 650 bases of the full-length sequence was used to examine Southern blots of DNA from normal persons and from a panel of mouse/human somatic cell hybrid lines. The pattern of hybridization of this cDNA to normal human DNA cut with 8 restriction endonucleases suggests the human genome has two (or more) P450cl7 genes. The pattern of hybridization to the somatic cell hybrid cell lines, each containing a limited, known number of human chromosomes, indicates the human adrenal P450cl7 gene lies on chromosome 10. The chromosomal locations of the other P450cl7 genes could not be determined.
All Science Journal Classification (ASJC) codes
- Endocrinology, Diabetes and Metabolism
- Clinical Biochemistry
- Biochemistry, medical