Assessment method for allelopathic effect from leaf litter leachates

Yoshiharu Fujii; Tomoko Shibuya; Keiko Nakatani; Tomio Itani; Syuntaro Hiradate; Mohammad M. Parvez

doi:10.1111/j.1445-6664.2003.00113.x

Assessment method for allelopathic effect from leaf litter leachates

Yoshiharu Fujii, Tomoko Shibuya, Keiko Nakatani, Tomio Itani, Syuntaro Hiradate, Mohammad M. Parvez

Research output: Contribution to journal › Article › peer-review

97 Citations (Scopus)

Abstract

In order to elucidate the allelopathic effect of leaf litter leachates under laboratory conditions, a modified 'sandwich method', which places leaves between two layers of agar, was used. Fifty mg of leaves was used per 10 cm ² cell. Agar concentrations at 0.5-1.0% were the best for gel support in determining radicle and hypocotyl elongation of lettuce. The optimum incubation time for bioassay was three days after imbibition onset. Among 20 typical tree species in Asia, Cymbopogon citratus and Derris scandens showed the strongest inhibitory activity determined by the sandwich method, followed by Piper betle, Tamarindus indica, and Gliricidia sepium. This bioassay seems to be a reliable method for screening allelopathic activity from leaf litter leachates.

Original language	English
Pages (from-to)	19-23
Number of pages	5
Journal	Weed Biology and Management
Volume	4
Issue number	1
DOIs	https://doi.org/10.1111/j.1445-6664.2003.00113.x
Publication status	Published - 2004
Externally published	Yes

All Science Journal Classification (ASJC) codes

Agronomy and Crop Science

Access to Document

10.1111/j.1445-6664.2003.00113.x

Cite this

@article{2e580e964e0d4cf58925584650b62999,

title = "Assessment method for allelopathic effect from leaf litter leachates",

abstract = "In order to elucidate the allelopathic effect of leaf litter leachates under laboratory conditions, a modified 'sandwich method', which places leaves between two layers of agar, was used. Fifty mg of leaves was used per 10 cm 2 cell. Agar concentrations at 0.5-1.0% were the best for gel support in determining radicle and hypocotyl elongation of lettuce. The optimum incubation time for bioassay was three days after imbibition onset. Among 20 typical tree species in Asia, Cymbopogon citratus and Derris scandens showed the strongest inhibitory activity determined by the sandwich method, followed by Piper betle, Tamarindus indica, and Gliricidia sepium. This bioassay seems to be a reliable method for screening allelopathic activity from leaf litter leachates.",

author = "Yoshiharu Fujii and Tomoko Shibuya and Keiko Nakatani and Tomio Itani and Syuntaro Hiradate and Parvez, {Mohammad M.}",

year = "2004",

doi = "10.1111/j.1445-6664.2003.00113.x",

language = "English",

volume = "4",

pages = "19--23",

journal = "Weed Biology and Management",

issn = "1444-6162",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "1",

}

TY - JOUR

T1 - Assessment method for allelopathic effect from leaf litter leachates

AU - Fujii, Yoshiharu

AU - Shibuya, Tomoko

AU - Nakatani, Keiko

AU - Itani, Tomio

AU - Hiradate, Syuntaro

AU - Parvez, Mohammad M.

PY - 2004

Y1 - 2004

N2 - In order to elucidate the allelopathic effect of leaf litter leachates under laboratory conditions, a modified 'sandwich method', which places leaves between two layers of agar, was used. Fifty mg of leaves was used per 10 cm 2 cell. Agar concentrations at 0.5-1.0% were the best for gel support in determining radicle and hypocotyl elongation of lettuce. The optimum incubation time for bioassay was three days after imbibition onset. Among 20 typical tree species in Asia, Cymbopogon citratus and Derris scandens showed the strongest inhibitory activity determined by the sandwich method, followed by Piper betle, Tamarindus indica, and Gliricidia sepium. This bioassay seems to be a reliable method for screening allelopathic activity from leaf litter leachates.

AB - In order to elucidate the allelopathic effect of leaf litter leachates under laboratory conditions, a modified 'sandwich method', which places leaves between two layers of agar, was used. Fifty mg of leaves was used per 10 cm 2 cell. Agar concentrations at 0.5-1.0% were the best for gel support in determining radicle and hypocotyl elongation of lettuce. The optimum incubation time for bioassay was three days after imbibition onset. Among 20 typical tree species in Asia, Cymbopogon citratus and Derris scandens showed the strongest inhibitory activity determined by the sandwich method, followed by Piper betle, Tamarindus indica, and Gliricidia sepium. This bioassay seems to be a reliable method for screening allelopathic activity from leaf litter leachates.

UR - http://www.scopus.com/inward/record.url?scp=1642376281&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642376281&partnerID=8YFLogxK

U2 - 10.1111/j.1445-6664.2003.00113.x

DO - 10.1111/j.1445-6664.2003.00113.x

M3 - Article

AN - SCOPUS:1642376281

SN - 1444-6162

VL - 4

SP - 19

EP - 23

JO - Weed Biology and Management

JF - Weed Biology and Management

IS - 1

ER -

Assessment method for allelopathic effect from leaf litter leachates

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this