TY - JOUR
T1 - Assay method for Escherichia coli photolyase activity using single-strand cis-syn cyclobutane pyrimidine dimer DNA as substrate
AU - Nakayama, Takuya
AU - Todo, Takeshi
AU - Notsu, Saori
AU - Nakazono, Manabu
AU - Zaitsu, Kiyoshi
PY - 2004/6/15
Y1 - 2004/6/15
N2 - A high-performance liquid chromatography method for the assay of Escherichia coli photolyase activity was developed. When cis-syn cyclobutane pyrimidine dimer was used as substrate, the Michaelis constant (Km) value for the photolyase activity was 100nM. The linear range of the calibration curve of the photolyase activity was 0.026-6.64μU/assay tube. The correlation coefficient for this linearity was 0.998. The limit of detection (S/N=3) was 26nU/assay tube. The photolyase activity was increased 1.6-fold in the presence of 5,10-methenyltetrahydrofolic acid in the enzyme reaction mixture.
AB - A high-performance liquid chromatography method for the assay of Escherichia coli photolyase activity was developed. When cis-syn cyclobutane pyrimidine dimer was used as substrate, the Michaelis constant (Km) value for the photolyase activity was 100nM. The linear range of the calibration curve of the photolyase activity was 0.026-6.64μU/assay tube. The correlation coefficient for this linearity was 0.998. The limit of detection (S/N=3) was 26nU/assay tube. The photolyase activity was increased 1.6-fold in the presence of 5,10-methenyltetrahydrofolic acid in the enzyme reaction mixture.
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U2 - 10.1016/j.ab.2004.03.021
DO - 10.1016/j.ab.2004.03.021
M3 - Article
C2 - 15158485
AN - SCOPUS:2542473570
SN - 0003-2697
VL - 329
SP - 263
EP - 268
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -