TY - JOUR
T1 - Ascidian sperm activating and attracting factor
T2 - Importance of sulfate groups for the activities and implication of its putative receptor
AU - Yoshida, Manabu
AU - Shiba, Kogiku
AU - Yoshida, Kaoru
AU - Tsuchikawa, Hiroshi
AU - Ootou, Ouichirou
AU - Oishi, Toru
AU - Murata, Michio
N1 - Funding Information:
We thank Y. Hosokawa, M. Sekifuji, and N. Sugii for their technical assistance. We also thank the directors and the staff of the Onagawa Field Science Center (Tohoku University) and International Coastal Research Center (University of Tokyo) for supplying materials for this study. The work was supported in part by the Uehara Memorial Foundation to M.Y. and Grants-in-Aid for Scientific Research from JSPS and MEXT, Japan (KAKENHI) to M.Y., T.O., and M.M.
PY - 2008/10/15
Y1 - 2008/10/15
N2 - The sperm activating and attracting factor (SAAF) from the eggs of the ascidian Ciona intestinalis was identified as the sulfated polyhydroxysterol,3α,4β,7α,26-tetrahydroxy-5α-cholestane-3,26-disulfate. We present a functional analysis of SAAF derivatives that reveals the roles of the various SAAF functional groups. Optical isomerism does not affect SAAF activities. Hydrolysis on one side, i.e. at the sulfate groups of SAAF, decreases the sperm-activating and sperm-attracting activities, while hydrolysis on both sides resulted in the loss of both activities. Biotinylated-SAAF lost its sperm-activating ability, but retained sperm-binding and chemotactic abilities. Thus, the sulfate groups of SAAF are responsible for these activities.
AB - The sperm activating and attracting factor (SAAF) from the eggs of the ascidian Ciona intestinalis was identified as the sulfated polyhydroxysterol,3α,4β,7α,26-tetrahydroxy-5α-cholestane-3,26-disulfate. We present a functional analysis of SAAF derivatives that reveals the roles of the various SAAF functional groups. Optical isomerism does not affect SAAF activities. Hydrolysis on one side, i.e. at the sulfate groups of SAAF, decreases the sperm-activating and sperm-attracting activities, while hydrolysis on both sides resulted in the loss of both activities. Biotinylated-SAAF lost its sperm-activating ability, but retained sperm-binding and chemotactic abilities. Thus, the sulfate groups of SAAF are responsible for these activities.
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U2 - 10.1016/j.febslet.2008.09.006
DO - 10.1016/j.febslet.2008.09.006
M3 - Article
C2 - 18789324
AN - SCOPUS:53049092876
SN - 0014-5793
VL - 582
SP - 3429
EP - 3433
JO - FEBS Letters
JF - FEBS Letters
IS - 23-24
ER -