@article{4eb495e25cb2489fae90166a59980678,
title = "Application of quantitative real-time PCR for monitoring the process of enrichment of clones on in vitro protein selection",
abstract = "In vitro selection of proteins from cDNA libraries using display technologies, such as the in vitro virus method, is a powerful means for the discovery of novel protein interactions. After iterative screening, selected proteins are usually identified and evaluated by cloning and sequencing analysis. Previously we applied real-time PCR for evaluation of the sequences obtained on in vitro virus screening. Here, we have presented additional data regarding monitoring of the process of enrichment of selected clones in each round of selection and elimination of false positives by real-time PCR, and have also discussed the utility of the novel method. This approach should also be applicable to other display technologies.",
author = "Kenichi Horisawa and Nobuhide Doi and Hideaki Takashima and Hiroshi Yanagawa",
note = "Funding Information: We wish to thank Dr. S. Tateyama, Dr. E. Miyamoto-Sato and Dr. M. Yonezawa for the useful suggestions during the preparation of the manuscript. This work was supported in part by a Grant-in-Aid for the 21st Century Center of Excellence (COE) Program entitled “Understanding and Control of Life{\textquoteright}s Function via Systems Biology (Keio University),” and special coordination funds from the Ministry of Education, Culture, Sports, Science and Technology of Japan, funds from the New Energy and Industrial Technology Development Organization (NEDO) of Japan, and a Grant-in-Aid for Research on Advanced Medical Technology, Health and Labor Sciences Research Grants from the Ministry of Health, Labor and Welfare of Japan.",
year = "2005",
month = feb,
doi = "10.1093/jb/mvi023",
language = "English",
volume = "137",
pages = "121--124",
journal = "Journal of biochemistry",
issn = "0021-924X",
publisher = "Oxford University Press",
number = "2",
}