Application of partially double-stranded DNA probes to high-throughput SNPs genotyping.

Tomoya Ishii; Kentaro Muraki; Naohiko Shimada; Arihiro Kano; Nao Nishida; Katsushi Tokunaga; Atsushi Maruyama

Application of partially double-stranded DNA probes to high-throughput SNPs genotyping.

Tomoya Ishii, Kentaro Muraki, Naohiko Shimada, Arihiro Kano, Nao Nishida, Katsushi Tokunaga, Atsushi Maruyama

Department of Fundamental Organic Chemistry

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We have focused on DNA strand exchange reaction (SER) with partially double-stranded (PDS) probes as a novel platform of SNPs genotyping. In this report, we elaborated the method for reliable and high-throughput SNPs typing. Competitive reactions using a couple of PDS probes designed for wild and mutant sequences significantly increase resolution efficiency to SNP types that is hardly discriminated by a non-competitive reaction with a single probe. Integration of the PDS probes with DNA microarray technology enable us to increase throughput efficacy. Simple and quick typing of fifteen SNPs with reliability as high as a ligase-based assay was demonstrated.

Original language	English
Pages (from-to)	237-238
Number of pages	2
Journal	Nucleic acids symposium series (2004)
Issue number	52
Publication status	Published - 2008

All Science Journal Classification (ASJC) codes

Medicine(all)

Cite this

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title = "Application of partially double-stranded DNA probes to high-throughput SNPs genotyping.",

abstract = "We have focused on DNA strand exchange reaction (SER) with partially double-stranded (PDS) probes as a novel platform of SNPs genotyping. In this report, we elaborated the method for reliable and high-throughput SNPs typing. Competitive reactions using a couple of PDS probes designed for wild and mutant sequences significantly increase resolution efficiency to SNP types that is hardly discriminated by a non-competitive reaction with a single probe. Integration of the PDS probes with DNA microarray technology enable us to increase throughput efficacy. Simple and quick typing of fifteen SNPs with reliability as high as a ligase-based assay was demonstrated.",

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AU - Muraki, Kentaro

AU - Shimada, Naohiko

AU - Kano, Arihiro

AU - Nishida, Nao

AU - Tokunaga, Katsushi

AU - Maruyama, Atsushi

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Y1 - 2008

N2 - We have focused on DNA strand exchange reaction (SER) with partially double-stranded (PDS) probes as a novel platform of SNPs genotyping. In this report, we elaborated the method for reliable and high-throughput SNPs typing. Competitive reactions using a couple of PDS probes designed for wild and mutant sequences significantly increase resolution efficiency to SNP types that is hardly discriminated by a non-competitive reaction with a single probe. Integration of the PDS probes with DNA microarray technology enable us to increase throughput efficacy. Simple and quick typing of fifteen SNPs with reliability as high as a ligase-based assay was demonstrated.

AB - We have focused on DNA strand exchange reaction (SER) with partially double-stranded (PDS) probes as a novel platform of SNPs genotyping. In this report, we elaborated the method for reliable and high-throughput SNPs typing. Competitive reactions using a couple of PDS probes designed for wild and mutant sequences significantly increase resolution efficiency to SNP types that is hardly discriminated by a non-competitive reaction with a single probe. Integration of the PDS probes with DNA microarray technology enable us to increase throughput efficacy. Simple and quick typing of fifteen SNPs with reliability as high as a ligase-based assay was demonstrated.

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JF - Nucleic acids symposium series (2004)

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Application of partially double-stranded DNA probes to high-throughput SNPs genotyping.

Abstract

All Science Journal Classification (ASJC) codes

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