Application of in vivo ESR/spin-probe technique to monitor tumor in vivo in mouse footpad

The redox status of tumors inoculated into the footpads of mice was investigated by using an in vivo ESR/spinprobe technique. A single-cell suspension of a metastatic subclone of colon carcinoma NL-17 was inoculated into the footpads of Balb/c mice. At 12, 24, 48, and 96 h after the inoculation, a spin probe, either carbamoyl-or carboxy-PROXYL, was intravenously injected, and then the ESR spectra of each footpad were separately obtained under a one-dimensional magnetic-field gradient. The change in the ESR signal intensity of the spin probe was closely related to the tumor volume in the footpads, but no significant difference was observed between carbamoyl- and carboxy-PROXYL. The in vivo ESR signal decay of carbamoyl-PROXYL, which is related to the conversion of the nitroxyl radical to hydroxylamine, was enhanced in the inoculated footpads but not in the reference one. The ESR signal decay was not influenced by coadministration of radical scavengers, SOD, catalase, mannitol, or dimethylthiourea, suggesting that the redox status but not reactive oxygen species generation played a role in the enhanced signal decay.