Application of immobilized lipase to regio-specific interesterification of triglyceride in organic solvent

Kenzo Yokozeki; Shigeru Yamanaka; Koichi Takinami; Yoshio Hirose; Atsuo Tanaka; Kenji Sonomoto; Saburo Fukui

doi:10.1007/BF00507995

Application of immobilized lipase to regio-specific interesterification of triglyceride in organic solvent

Kenzo Yokozeki, Shigeru Yamanaka, Koichi Takinami, Yoshio Hirose, Atsuo Tanaka, Kenji Sonomoto, Saburo Fukui

Research output: Contribution to journal › Article › peer-review

163 Citations (Scopus)

Abstract

Lipase from Rhizopus delemar was immobilized by entrapment with photo-crosslinkable resin prepolymers or urethane prepolymers or by binding to various types of porous silica beads. The immobilized lipase preparations thus obtained were examined for their activity in converting olive oil to an interesterified fat (cacao butter-like fat), whose oleic acid moieties at 1- and 3-positions were replaced with stearic acid moieties, in the reaction solvent n-hexane. Although all of the immobilized preparations exhibited some activity, lipase adsorbed on Celite and then entrapped with a hydrophobic photo-crosslinkable resin prepolymer showed the highest activity, about 75% of that of lipase simply adsorbed onto Celite. Entrapment markedly enhanced the operational stability of lipase.

Original language	English
Pages (from-to)	1-5
Number of pages	5
Journal	European Journal of Applied Microbiology and Biotechnology
Volume	14
Issue number	1
DOIs	https://doi.org/10.1007/BF00507995
Publication status	Published - Mar 1982
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Microbiology

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abstract = "Lipase from Rhizopus delemar was immobilized by entrapment with photo-crosslinkable resin prepolymers or urethane prepolymers or by binding to various types of porous silica beads. The immobilized lipase preparations thus obtained were examined for their activity in converting olive oil to an interesterified fat (cacao butter-like fat), whose oleic acid moieties at 1- and 3-positions were replaced with stearic acid moieties, in the reaction solvent n-hexane. Although all of the immobilized preparations exhibited some activity, lipase adsorbed on Celite and then entrapped with a hydrophobic photo-crosslinkable resin prepolymer showed the highest activity, about 75% of that of lipase simply adsorbed onto Celite. Entrapment markedly enhanced the operational stability of lipase.",

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AU - Yokozeki, Kenzo

AU - Yamanaka, Shigeru

AU - Takinami, Koichi

AU - Hirose, Yoshio

AU - Tanaka, Atsuo

AU - Sonomoto, Kenji

AU - Fukui, Saburo

PY - 1982/3

Y1 - 1982/3

N2 - Lipase from Rhizopus delemar was immobilized by entrapment with photo-crosslinkable resin prepolymers or urethane prepolymers or by binding to various types of porous silica beads. The immobilized lipase preparations thus obtained were examined for their activity in converting olive oil to an interesterified fat (cacao butter-like fat), whose oleic acid moieties at 1- and 3-positions were replaced with stearic acid moieties, in the reaction solvent n-hexane. Although all of the immobilized preparations exhibited some activity, lipase adsorbed on Celite and then entrapped with a hydrophobic photo-crosslinkable resin prepolymer showed the highest activity, about 75% of that of lipase simply adsorbed onto Celite. Entrapment markedly enhanced the operational stability of lipase.

AB - Lipase from Rhizopus delemar was immobilized by entrapment with photo-crosslinkable resin prepolymers or urethane prepolymers or by binding to various types of porous silica beads. The immobilized lipase preparations thus obtained were examined for their activity in converting olive oil to an interesterified fat (cacao butter-like fat), whose oleic acid moieties at 1- and 3-positions were replaced with stearic acid moieties, in the reaction solvent n-hexane. Although all of the immobilized preparations exhibited some activity, lipase adsorbed on Celite and then entrapped with a hydrophobic photo-crosslinkable resin prepolymer showed the highest activity, about 75% of that of lipase simply adsorbed onto Celite. Entrapment markedly enhanced the operational stability of lipase.

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Application of immobilized lipase to regio-specific interesterification of triglyceride in organic solvent

Abstract

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