Application of HTS SQUIDs to biological immunoassays

Keiji Enpuku; Akihiro Ohba; Katsuhiro Inoue; Tong Qing Yang

doi:10.1016/j.physc.2003.12.098

Application of HTS SQUIDs to biological immunoassays

Keiji Enpuku, Akihiro Ohba, Katsuhiro Inoue, Tong Qing Yang

Superconductive Systems Engineering Laboratory

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Biological binding-reaction between an antigen and its antibody was detected by using a magnetic marker and a high T_c superconducting quantum interference device (SQUID). In this method, the binding reaction is detected by measuring the magnetic field from the marker. A new marker made of Fe₃O₄ particle with diameter of 25 nm was developed, and the remanent field of the marker was used for the detection of the antigen called Interleukin 8 (IL8). It was shown that the present system can detect 0.1 pg weight of IL8. Issues for the improvement of the system performance were also discussed, such as binding efficiency of the marker to IL8 and the dependence of the signal flux on the geometrical parameters of the sample and the SQUID.

Original language	English
Pages (from-to)	1473-1479
Number of pages	7
Journal	Physica C: Superconductivity and its applications
Volume	412-414
Issue number	SPEC. ISS.
DOIs	https://doi.org/10.1016/j.physc.2003.12.098
Publication status	Published - Oct 2004

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Energy Engineering and Power Technology
Electrical and Electronic Engineering

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10.1016/j.physc.2003.12.098

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title = "Application of HTS SQUIDs to biological immunoassays",

abstract = "Biological binding-reaction between an antigen and its antibody was detected by using a magnetic marker and a high Tc superconducting quantum interference device (SQUID). In this method, the binding reaction is detected by measuring the magnetic field from the marker. A new marker made of Fe3O4 particle with diameter of 25 nm was developed, and the remanent field of the marker was used for the detection of the antigen called Interleukin 8 (IL8). It was shown that the present system can detect 0.1 pg weight of IL8. Issues for the improvement of the system performance were also discussed, such as binding efficiency of the marker to IL8 and the dependence of the signal flux on the geometrical parameters of the sample and the SQUID.",

author = "Keiji Enpuku and Akihiro Ohba and Katsuhiro Inoue and Yang, {Tong Qing}",

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T1 - Application of HTS SQUIDs to biological immunoassays

AU - Enpuku, Keiji

AU - Ohba, Akihiro

AU - Inoue, Katsuhiro

AU - Yang, Tong Qing

PY - 2004/10

Y1 - 2004/10

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AB - Biological binding-reaction between an antigen and its antibody was detected by using a magnetic marker and a high Tc superconducting quantum interference device (SQUID). In this method, the binding reaction is detected by measuring the magnetic field from the marker. A new marker made of Fe3O4 particle with diameter of 25 nm was developed, and the remanent field of the marker was used for the detection of the antigen called Interleukin 8 (IL8). It was shown that the present system can detect 0.1 pg weight of IL8. Issues for the improvement of the system performance were also discussed, such as binding efficiency of the marker to IL8 and the dependence of the signal flux on the geometrical parameters of the sample and the SQUID.

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Application of HTS SQUIDs to biological immunoassays

Abstract

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