TY - JOUR
T1 - Antiviral effects against influenza a virus infection by a short hairpin rna targeting the non-coding terminal region of the viral nucleoprotein gene
AU - Fujimoto, Yoshikazu
AU - Kyogoku, Kenji
AU - Takeda, Keiko
AU - Ozaki, Kinuyo
AU - Yamamoto, Sayo
AU - Suyama, Haruka
AU - Ono, Etsuro
N1 - Funding Information:
ACKNOWLEDGMENTS. We are grateful to Dr. Hirokazu Sakamoto (Frontier Biomedical & Medical Research Laboratories, Kaneka Corporation) for useful discussions and advice on this work. This work was financially supported by JSPS KAKENHI Grant Number 16K21214.
Publisher Copyright:
© 2019 The Japanese Society of Veterinary Science.
PY - 2019/3
Y1 - 2019/3
N2 - RNA interference (RNAi) can inhibit Influenza A virus (IAV) infection in a gene-specific manner. In this study, we constructed a transgene expressing a short hairpin RNA (shRNA) that targets the noncoding region of the IAV RNA gene encoding nucleoprotein (NP). To investigate the antiviral effects of the shRNA, we generated two transgenic mouse lines with this transgene. Unfortunately, there was no apparent difference in IAV resistance between transgenic and non-transgenic littermates. To further investigate the antiviral effects of the shRNA, we prepared mouse embryonic fibroblasts (MEFs) from transgenic and non-transgenic mice. In experimental infections using these MEFs, virus production of mouse-adapted IAV strain A/Puerto Rico/8/1934 (PR8) in the transgenic MEFs was suppressed by means of the down-regulation of the viral RNA gene transcription in the early stages of infection in comparison with non-transgenic MEFs. These results indicated that expression of the shRNA was able to confer antiviral properties against IAVs to MEFs, although the effects were limited. Our findings suggest that the shRNA targeting the noncoding region of the viral RNA (vRNA) of NP might be a supporting tool in developing influenza-resistant poultry.
AB - RNA interference (RNAi) can inhibit Influenza A virus (IAV) infection in a gene-specific manner. In this study, we constructed a transgene expressing a short hairpin RNA (shRNA) that targets the noncoding region of the IAV RNA gene encoding nucleoprotein (NP). To investigate the antiviral effects of the shRNA, we generated two transgenic mouse lines with this transgene. Unfortunately, there was no apparent difference in IAV resistance between transgenic and non-transgenic littermates. To further investigate the antiviral effects of the shRNA, we prepared mouse embryonic fibroblasts (MEFs) from transgenic and non-transgenic mice. In experimental infections using these MEFs, virus production of mouse-adapted IAV strain A/Puerto Rico/8/1934 (PR8) in the transgenic MEFs was suppressed by means of the down-regulation of the viral RNA gene transcription in the early stages of infection in comparison with non-transgenic MEFs. These results indicated that expression of the shRNA was able to confer antiviral properties against IAVs to MEFs, although the effects were limited. Our findings suggest that the shRNA targeting the noncoding region of the viral RNA (vRNA) of NP might be a supporting tool in developing influenza-resistant poultry.
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U2 - 10.1292/jvms.18-0436
DO - 10.1292/jvms.18-0436
M3 - Article
C2 - 30674743
AN - SCOPUS:85062858701
SN - 0916-7250
VL - 81
SP - 383
EP - 388
JO - Journal of Veterinary Medical Science
JF - Journal of Veterinary Medical Science
IS - 3
ER -