Antioxidant Activity and Cytotoxicity of the Traditional Indonesian Medicine Tahongai (Kleinhovia hospita L.) Extract

Enos Tangke Arung, Irawan Wijaya Kusuma, Sri Purwatiningsih, Seong Soo Roh, Chae Ha Yang, Soohyeon Jeon, Yong Ung Kim, Edi Sukaton, Joko Susilo, Yuli Astuti, Britanto Dani Wicaksono, Ferry Sandra, Kuniyoshi Shimizu, Ryuichiro Kondo

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


We investigated the leaves of Kleinhovia hospita, a plant which has been traditionally used in Indonesia as phytotherapy to cure liver disease, to describe antioxidant materials from plant sources. K. hospita leaves were extracted with methanol and further partitioned into n-hexane, diethyl ether, and ethyl acetate. The antioxidant activity of each fraction and the residue was assessed using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method and their cytotoxicity on HepG2 liver cancer cells was determined by a MTT assay. The K. hospita leaf methanol extract showed strong antioxidant activity (96%) compared with vitamin C (98%) by the DPPH method and the measured activity from the subsequent extracts of the methanol extract were 48.9% for n-hexane, 74.0% for diethyl ether, 84.3% for ethyl acetate, and 77.1% for the residue. The MTT assay showed the cytotoxicity of the methanol extract on HepG2 cells at 14%, 76%, and 80% at concentrations of 50μg/mL, 87.5μg/mL, and 125μg/mL, respectively. Leaf extracts of the medicinal plant K. hospita showed potent antioxidant activity and moderate cytotoxicity on HepG2 liver cancer cells.

Original languageEnglish
Pages (from-to)306-308
Number of pages3
JournalJAMS Journal of Acupuncture and Meridian Studies
Issue number4
Publication statusPublished - Dec 2009

All Science Journal Classification (ASJC) codes

  • Complementary and alternative medicine
  • Anesthesiology and Pain Medicine


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