Antimicrobial effects of pressured carbon dioxide in a continuous flow system

M. Shimoda, Y. Yamamoto, J. Cocunubo-Castellanos, H. Tonoike, T. Kawano, H. Ishikawa, Y. Osajima

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77 Citations (Scopus)


The application of microbubbles of pressured CO2 greatly increased CO2 concentration in the solution treated. By treatment at 6 MPa, 35°C and average residence time 15 min, L. brevis was completely inactivated at the level of dissolved CO2, γ ≥11 (γ, Kuenen's gas absorption coefficient). E. coli and S. cerevisiae required γ ≥ 17, and T. versatilis required γ ≥ 21 for complete inactivation. Z. rouxii could be sterilized at 20 MPa and 26. A comparison of the continuous and batch method showed that L. brevis was inactivated completely under pressured CO2>0.16 g/cm3 with the continuous method and >0.9 g/cm3 with the batch method.

Original languageEnglish
Pages (from-to)709-712
Number of pages4
JournalJournal of Food Science
Issue number4
Publication statusPublished - 1998

All Science Journal Classification (ASJC) codes

  • Food Science


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