Antigen-specific in vitro immunization: A source for human monoclonal antibodies

Kosuke Tomimatsu, Sanetaka Shirahata

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)


Human monoclonal antibody has great potential for treatment of various diseases utilizing their specificity against antigens. We have shown an in vitro immunization (IVI) protocol inducing antigen-specific immune responses in human peripheral blood mononuclear cells (PBMCs) for efficient production of human monoclonal antibodies. By using IVI method antigen specific antibody genes can be efficiently obtained because of increasing production of antigen-specific antibodies from in vitro immunized PBMCs. This IVI protocol will be widely applied for combination with several display methods and enhance the production of human monoclonal antibodies.

Original languageEnglish
Title of host publicationHuman Monoclonal Antibodies
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages11
ISBN (Print)9781627035859
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics


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