Antibacterial activity of ammonium hexafluorosilicate solution with antimicrobial agents for the prevention of dentin caries

Purpose: This study evaluated the antibacterial activity of the SiF solution with the addition of antibacterial agents on a Streptococcus mutans biofilm. Methods: Various antibacterial SiF solutions were prepared by adding chlorhexidine, cetylpyridinium chloride, isopropyl methylphenol, or epigallocatechin gallate. Hydroxyapatite pellets treated with several SiF solutions were immersed in BHI inoculated with S. mutans standardized suspension. The number of S. mutans cells adhered to each pellet was evaluated. Results: SiF with the addition of CPC was the most effective for reducing the adherence of bacteria and inhibiting the formation of biofilm, showing the same level as AgF, In contrast, the addition of other antibacterial agents to SiF reduced the original antibacterial activity of SiF solution.