TY - JOUR
T1 - Anti-proliferative and Apoptotic Activity Against an Acute Myeloid Leukemia Cell Line by Constituents from Ficus benghalensis
AU - Hassan, Heba Ali
AU - Allam, Ahmed E.
AU - Alaaeldin, Rania
AU - Mustafa, Muhamad
AU - Mohyeldin, Reham H.
AU - Shimizu, Kuniyoshi
AU - Abdelwahab, Sayed F.
AU - Desouky, Samar Y.
AU - Mohamed, Khaled M.
AU - Kamel, Mohamed S.
N1 - Funding Information:
Taif University Researchers Supporting Project (number TURSP-2020/51), Taif University, Taif, Saudi Arabia.
Publisher Copyright:
© 2022, The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia.
PY - 2022/8
Y1 - 2022/8
N2 - Phytochemical investigation of Ficus benghalensis L., Moraceae, leaves revealed three known compounds, carpachromene, mucusoside, and 2-O-α-l-rhamnopyranosyl-(2R)-hydroxyhexacosanoate-β-d-glucopyranosyl ester. Herein, the cytotoxic activity of the isolated compounds was measured against the HL60 cancer cell line. Our findings revealed that carpachromene exerted potent anti-proliferative activity. Also, this compound induced apoptosis and cell cycle arrest at the G2/M phase. Docking analysis revealed the potential interaction of carpachromene with topoisomerase I. This was confirmed by western blotting where carpachromene inhibited topoisomerase I protein expression. Additionally, carpachromene upregulated the expression of pro-apoptotic genes. In conclusion, F. benghalensis leaves exerted potent anti-proliferative activity against HL60 cancer cells. [Figure not available: see fulltext.]
AB - Phytochemical investigation of Ficus benghalensis L., Moraceae, leaves revealed three known compounds, carpachromene, mucusoside, and 2-O-α-l-rhamnopyranosyl-(2R)-hydroxyhexacosanoate-β-d-glucopyranosyl ester. Herein, the cytotoxic activity of the isolated compounds was measured against the HL60 cancer cell line. Our findings revealed that carpachromene exerted potent anti-proliferative activity. Also, this compound induced apoptosis and cell cycle arrest at the G2/M phase. Docking analysis revealed the potential interaction of carpachromene with topoisomerase I. This was confirmed by western blotting where carpachromene inhibited topoisomerase I protein expression. Additionally, carpachromene upregulated the expression of pro-apoptotic genes. In conclusion, F. benghalensis leaves exerted potent anti-proliferative activity against HL60 cancer cells. [Figure not available: see fulltext.]
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U2 - 10.1007/s43450-022-00267-5
DO - 10.1007/s43450-022-00267-5
M3 - Article
AN - SCOPUS:85131593684
SN - 0102-695X
VL - 32
SP - 527
EP - 536
JO - Revista Brasileira de Farmacognosia
JF - Revista Brasileira de Farmacognosia
IS - 4
ER -
